Biolegend, 147104, PE anti-mouse CD300LG (Nepmucin) Antibody, 100μg

CD300LG, also known as nepmucin (a not expressed in Peyer's patches mucin), is a member of the CD300 antigen like family. It is a 50-95 kD glycoprotein with one IgV like domain and one mucin like domain and four isoforms are produced by alternative splicing. Nepmucin is expressed on high endothelial venules (HEV) from lymph nodes, but is absent on HEV from the Peyer's patches. CD300LG mediates lymphocyte adhesion through the intraction of its Ig domain with ICAM-1 and rolling on HEVs through the interaction of its mucin like domain with L-selectine. Also, it may play a role in the molecular transcytosis across the capillary endothelium.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Nepmucin-Fc fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications for the relevant formats include: immunoelectron microscopy of peripheral LN HEVs1.
Application References(PubMed link indicates BioLegend citation): Umemoto E, et al. 2006. J. Exp. Med. 203:1603. (EM) Jin S, et al. 2008. FEBS Lett. 582:3018. Umemoto E, et al. 2011. Crit. Rev. Immunol. 31:147. Umemoto E, et al. 2013. PLoS One 8:e83681.
RRID: AB_2562971 (BioLegend Cat. No. 147104)
Structure: Sialomucin, member of the CD300 antigen like family, 50-95 kD. 1 IgV like domain, 1 mucin like domain. Four isoforms produced by alternative splicing.
Distribution: Expressed on high endothelial venules (HEVs) from lymph nodes but absent on HEVs from Peyer's patches, some myeloid cell lines.
Function: Mediator of lymphocyte adhesion and rolling on HEVs, possible role in transcytosis across the capillary endothelium.
Ligand/Receptor: L-selectin.
Biology Area: Cell Adhesion, Cell Biology, Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Takatsu H, et al. 2006. Biochem. Biophys. Res. Commun. 348:183. 2. Umemoto E, et al. 2006. J. Exp. Med. 203:1603. 3. Umemoto E, et al. 2011. Crit. Rev. Immunol. 31:147.
Gene ID: 52685
UniProt: View information about CD300LG on UniProt.org
Clone: ZAQ5
Regulatory Status: RUO
Other Names: CMRF35-like molecule 9, CLM-9, CD300g, D11Ertd736e
Isotype: Rat IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.