Biolegend, 147402, Purified anti-mouse GITR Ligand Antibody, 100μg

GITR ligand (GITRL) is a member of the TNF superfamily, also known as TNFSF18 or TL6. It is expressed on B cells, macrophages, dendritic cells, and endothelial cells. The expression of GITRL on the dendritic cell surface can be transiently upregulated, and then rapidly downregulated, by LPS stimulation. GITRL binds to its receptor, GITR, to costimulate T cell activation, proliferation, and cytokine production, inhibit the suppressive functions of CD4 + CD25 + Tregs, and induce macrophage activation. GITR-GITRL interaction has been implicated as an essential regulator of the immune response to self antigens and phathogens.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additonal reported applications (for relevant formats of thes clone) include: inhibition of GITR-GITRL mediated T cell proliferation and reversing Treg-mediated suppression1.The following patents apply to clone 5F1: U.S. Patent Serial No. 7,618,632 and Japan Patent Serial No. 4638876.
Application References(PubMed link indicates BioLegend citation): Stephens GL, et al. 2004. J. Immunol. 173:5008. (Block)
RRID: AB_2563295 (BioLegend Cat. No. 147402)
Structure: TNF superfamily member
Distribution: B cells, macrophages, dendritic cells, endothelial cells
Function: Binds GITR receptor to costimulate T cell activation, proliferation, and cytokine production; inhibits the suppressive activities of CD4+CD25+ Tregs
Cell Type: B cells, Macrophages, Dendritic cells, Endothelial cells, Tregs
Biology Area: Immunology
Antigen References: 1. Tone M, et al. 2003. Proc. Natl. Acad. Sci. USA 100:15059. 2. Kim JD, et al. 2003. Genes Immun. 4:564. 3. Yu KY, et al. 2003. Biochem. Biophys. Res. Commun. 310:433. 4. Shin HH, et al. 2002. Cytokine 19:187.
Gene ID: 240873
UniProt: View information about GITR Ligand on UniProt.org
Clone: 5F1
Regulatory Status: RUO
Other Names: GITRL, AITRL, TNFSF18, TL6
Isotype: Rat IgG2a, κ