Biolegend, 147604, PE anti-mouse CD100 (SEMA4D) Antibody, 100μg

CD100, also known as Semaphorin-4D (SEMA4D), is a 150 kD, type I transmembrane protein, with one sema domain and one C2-type Ig domain. It is found as a homodimer on the cell surface. CD100 is expressed in the brain, kidney, heart, the majority of hematopoietic cells, and some malignant cells. CD100 is a potent inducer of angiogenesis and inhibitor of bone formation, and plays a role in lymphocyte activation, antibody production, dendritic cell maturation, and axon guidance. Its ligands are CD72 and Plexin-B1. CD100 is cleaved from the membrane of activated T and B cells, producing a soluble form.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse CD100-Fc fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications for the relevant formats include: immunoprecipitation from cell lysates2, capture antibody function in a sandwich ELISA assay2, and immunohistochemical staining of frozen brain sections4.
Application References(PubMed link indicates BioLegend citation): Kumanogoh A, et al. 2000. Immunity. 13:621. (FC) Wang X, et al. 2001. Blood. 97:3498. (FC, IP, ELISA) Kumanogoh A, et al. 2002. J. Immunol. 169:1175. (FC) Moreau-Fauvarque C, et al. 2003 J. Neurosci. 23:9229. (IHC)
Product Citations: Borrelli C, et al. 2024. Nature. 411:632. PubMed
RRID: AB_2563613 (BioLegend Cat. No. 147603)
Structure: Type I transmembrane protein, one sema domain, one C2-type immunoglobulin domain, homodimer, 150 kD.
Distribution: Highly expressed on most hematopoietic cells, is also found in the brain, kidney, and heart. Overexpressed by some malignant cell types.
Function: Potent inducer of angiogenesis, inhibitor of bone formation, plays a role in lymphocyte activation, antibody production and dendritic cell maturation. In neurons, regulates dendrite branching and axon guidance.
Ligand/Receptor: CD72, Plexin-B1.
Cell Type: B cells, Dendritic cells, Neutrophils, Tregs
Biology Area: Angiogenesis, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Neuroscience, Signal Transduction, Synaptic Biology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85. 3. Kishihara K, et al. 1993. Cell. 74:143. 4. Pulido R, et al. 1988. J. Immunol. 140:3851.
Gene ID: 20354
UniProt: View information about CD100 on UniProt.org
Clone: BMA-12
Regulatory Status: RUO
Other Names: Neuroimmune semaphorin 4D, Sema4D, M-Sema G, Semaphorin-C-like 2, Semaphorin-J, Semaj, Semcl2, Coll-4, Semacl2
Isotype: Rat IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.