Biolegend, 148004, PE anti-mouse CD300c/d Antibody, 100μg

CD300 receptors are a group of type I transmembrane proteins, which belong to the Ig superfamily. In mice, nine members have been reported with different nomenclature systems, such as myeloid-associated Ig-like receptor (MAIR), leukocyte mono-Ig-like receptor (LMIR), DC-derived Ig-like receptor (DIgR), and CMRF-35-like molecule (CLM). The CD300 molecules act as either activating or inhibitory receptors through interactions with adaptor molecules, such as PAD12, DAP10, and FcRγ. Despite sharing high homology with CD300a, CD300c (LMIR-II) does not share its inhibitory function. CD300c has been found to activate a variety of cell types and induce proinflammatory molecule production. CD300d may be involved with the regulation/formation of CD300 complexes on the cell surface.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: MAIR-II transfected Ba/F3
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: Western blotting1.
RRID: AB_2563674 (BioLegend Cat. No. 148003) AB_2563675 (BioLegend Cat. No. 148004)
Structure: Type I transmemrane protein, Ig superfamily
Distribution: B cell subsets, macrophages, dendritic cells
Interaction: DAP12, DAP10, FcRγ
Ligand/Receptor: Phosphatidylserine
Cell Type: B cells, Macrophages, Dendritic cells
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules
Antigen References: 1. Yotsumoto K, et al. 2003. J. Exp. Med. 198:223.2. Martinez-Barriocanal A, et al. 2010. J. Biol. Chem. 285:41781.3. Borrego F. 2013. Blood. 121:1951.4. Lankry D, et al. 2013. Eur. J. Immunol. 43:2151.
Gene ID: 387565140497
UniProt: View information about CD300c/d on UniProt.org
Clone: TX52
Regulatory Status: RUO
Other Names: CD300c/d, MAIR-II, LMIR-II, CLM-4, DIgR1, CLM-6
Isotype: Rat IgG2b, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.