Biolegend, 148203, PE anti-mouse/rat XCR1 Antibody, 25μg

XCR1, also known as GPR5 or CCXCR1, is a 38 kD, G-protein coupled, seven transmembrane receptor, and the only member of the "C" chemokine receptor family. XCR1 mediates chemotaxis of XCL1 and XCL2 (lymphotactin-1 and -2), and defines a subset of CD8α + conventional dendritic cells capable of antigen cross-presentation.
25μg
Verified Reactivity: Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining on frozen tissues2.
Application References(PubMed link indicates BioLegend citation): Gurka S, et al. 2015. Front Immunol. 6:35. (FC) Kitazawa Y, et al. 2019. Front Immunol. 10:1195. (FC, IHC-F)
Product Citations: Wu R, et al. 2022. Nat Immunol. 23:1536. PubMed van Elsas MJ, et al. 2023. J Immunother Cancer. 11:. PubMed Kim S, et al. 2020. Immunity. 53(4):759-774.e9. PubMed Van Den Eeckhout B, et al. 2020. NPJ Vaccines. 5:64. PubMed Gotoh K et al. 2018. Cell reports. 25(7):1800-1815 . PubMed Niss K, et al. 2020. Cell Reports. 31(11):107763. PubMed Canton J, et al. 2021. Nat Immunol. 22:140. PubMed Leylek R, et al. 2019. Cell Rep. 29:3736. PubMed , et al. 2021. Eur J Immunol. 51:2708. PubMed de Reuver R, et al. 2021. Cell Reports. 36(6):109500. PubMed Bagadia P, et al. 2019. Nat Immunol. 20:1174. PubMed Sharma MD, et al. 2018. Immunity. 48:91. PubMed Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed Van Den Eeckhout B, et al. 2020. NPJ Vaccines. 0.252777778. PubMed Lin H, et al. 2021. Cancer Cell. 39:480. PubMed Freed-Pastor WA, et al. 2021. Cancer Cell. 39:1342. PubMed Hildreth AD, et al. 2020. STAR Protoc. 1:100113. PubMed Bosteels C, et al. 2020. Immunity. 52(6):1039-1056.e9.. PubMed Huang X, et al. 2021. Immunity. . PubMed C Khouili S, et al. 2020. Cell Rep. 33:108468. PubMed
RRID: AB_2563842 (BioLegend Cat. No. 148203) AB_2563843 (BioLegend Cat. No. 148204)
Structure: Only member of the "C" chemokine receptor family, seven transmembrane receptor, coupled to G protein, 38 kD.
Distribution: Subset of CD8α+ conventional dendritic cells.
Function: Mediates chemotaxis to XCL1, defines a population of CD8α+ dendritic cells capable of antigen cross-presentation to CD8+ T cells.
Ligand/Receptor: XCL1 and XCL2.
Cell Type: Dendritic cells
Biology Area: Cell Biology, Immunology, Innate Immunity, Signal Transduction
Molecular Family: Cytokine/Chemokine Receptors, GPCR
Antigen References: 1. Yamazaki C, et al. 2013. J. Immunol. 190:6071. 2. Shimizu K, et al. 2013. J. Immunol. 190:5609. 3. Bachem A, et al. 2012. Front. Immunol. 3:214. 4. Crozat K, et al. 2011. J. Immunol. 187:4411.
Gene ID: 23832301086
UniProt: View information about XCR1 on UniProt.org
Clone: ZET
Regulatory Status: RUO
Other Names: GPR5, CCXCR1, mXCR1, Lymphotactin receptor, SCM1 receptor, XC chemokine receptor 1
Isotype: Mouse IgG2b, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.