Biolegend, 148214, Alexa Fluor® 647 anti-mouse/rat XCR1 Antibody, 100μg

XCR1, also known as GPR5 or CCXCR1, is a 38 kD, G-protein coupled, seven transmembrane receptor, and the only member of the "C" chemokine receptor family. XCR1 mediates chemotaxis of XCL1 and XCL2 (lymphotactin-1 and -2), and defines a subset of CD8α + conventional dendritic cells capable of antigen cross-presentation.
100μg
Verified Reactivity: Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining on frozen tissues2.
Application References(PubMed link indicates BioLegend citation): Gurka S, et al. 2015. Front Immunol. 6:35. (FC) Kitazawa Y, et al. 2019. Front Immunol. 10:1195. (FC, IHC-F)
Product Citations: Frederico B, et al. 2022. Dev Cell. 57:1957. PubMed Uchil PD et al. 2018. Cell host & microbe. 25(1):87-100 . PubMed Liu D et al. 2019. Immunity. 51(1):64-76 . PubMed Dahlgren MW et al. 2019. Immunity. 50(3):707-722 . PubMed Bonavita E, et al. 2020. Immunity. 1215:53. PubMed Sen D, et al. 2016. PLoS One. 11:e0165064. PubMed
RRID: AB_2564368 (BioLegend Cat. No. 148213) AB_2564369 (BioLegend Cat. No. 148214)
Structure: Only member of the "C" chemokine receptor family, seven transmembrane receptor, coupled to G protein, 38 kD.
Distribution: Subset of CD8α+ conventional dendritic cells.
Function: Mediates chemotaxis to XCL1, defines a population of CD8α+ dendritic cells capable of antigen cross-presentation to CD8+ T cells.
Ligand/Receptor: XCL1 and XCL2.
Cell Type: Dendritic cells
Biology Area: Cell Biology, Immunology, Innate Immunity, Signal Transduction
Molecular Family: Cytokine/Chemokine Receptors, GPCR
Antigen References: 1. Yamazaki C, et al. 2013. J. Immunol. 190:6071. 2. Shimizu K, et al. 2013. J. Immunol. 190:5609. 3. Bachem A, et al. 2012. Front. Immunol. 3:214. 4. Crozat K, et al. 2011. J. Immunol. 187:4411.
Gene ID: 23832301086
UniProt: View information about XCR1 on UniProt.org
Clone: ZET
Regulatory Status: RUO
Other Names: GPR5, CCXCR1, mXCR1, Lymphotactin receptor, SCM1 receptor, XC chemokine receptor 1
Isotype: Mouse IgG2b, κ