Biolegend, 148508, PerCP/Cyanine5.5 anti-mouse/rat CD42d Antibody, 100μg

CD42d is an 83 kD surface glycoprotein that non-covalently associates with GPIb and GPIX to form a receptor complex for von Willebrand factor on megakaryocytes and resting platelets. Binding sites for von Willebrand factor and thrombin have been localized to the GPIba chain of the GPI-b-V-IX complex. Platelet activation with thrombin cleaves the GPI-b-V-IX complex to release a 69 kD soluble fragment. Presence of the GPI-b-V-IX complex is important in Bernard-Soulier syndrome, a rare bleeding disorder.
100μg
Verified Reactivity: Mouse, Rat
Antibody Type: Monoclonal
Host Species: Armenian Hamster
Immunogen: Mouse platelets
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for relevant formats) include: immunoprecipitation1,3.
Application References(PubMed link indicates BioLegend citation): Takada K, et al. 1995. Hybridoma. 14:361. (IP) Saito M, et al. 1996. Stem Cells. 14:124. (FC) Sato N, et al. 2000. Hybridoma. 19:455. (IP)
Product Citations: Zhang H, et al. 2023. Pharmaceutics. 15:. PubMed
RRID: AB_2564603 (BioLegend Cat. No. 148507) AB_2564604 (BioLegend Cat. No. 148508)
Structure: 83 kD heterodimer composed of 2 polypeptide chains, an α chain (GP1BA) and a β chain (GP1BB).
Distribution: Resting platelets and megakaryocytes.
Function: CD42d is a component of the GPIb-V-IX complex, functions as a vWF receptor, and mediates vWF-dependent platelet adhesion to blood vessels.
Ligand/Receptor: CD42d.
Cell Type: Megakaryocytes, Platelets
Biology Area: Cell Adhesion, Cell Biology, Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Sivaraman B, et al. 2011. Biomaterials. 32:5365. 2. Berger G, et al. 1996. Blood. 87:1385. 3. Ravanat C, et al. 1997. Blood. 89:3253. 4. Andrews RK, et al. 1998. Biochemistry. 37:638. 5. Inoue O, et al. 2008. 283:16279. 6. Hickey MJ, et al. 1993. Proc. Natl. Acad. Sci. 90:8327. 7. Shimomura T, et al. 1990. Blood. 75:2349. 8. Wu G, et al. 1997. Blood. 90:2660.
Gene ID: 14729
UniProt: View information about CD42d on UniProt.org
Clone: 1C2
Regulatory Status: RUO
Other Names: Platelet glycoprotein V, GPV, platelet gpV, CD42d antigen, CD42d
Isotype: Armenian Hamster IgG
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.