Biolegend, 148602, Purified anti-mouse CD282 (TLR2) Antibody, 500μg

CD282, also known as TLR2, is an 89 kD, type I transmembrane protein, with 14 leucine-rich repeats (LRR) in the extracellular region and one toll/IL-1R (TIR) domain in the intracellular region. TLR2 is mainly expressed on monocytes, macrophages, dendritic cells, granulocytes, and endothelial and epithelial cells. TLR2 associates with TLR1 and TLR6, and mediates the innate immune response against several components of Gram-positive bacteria, such as peptidoglycan, lipoteichoic acid (LTA), lipoarabinomanan, and lipoproteins as well as different LPS from certain Gram-negative bacteria, yeast, spirochetes, and fungi.
500μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse TLR2-transfected Ba/F3 cells.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: 1. Shibata T, et al. 2012. Int. Immunol. 24:613. (FC)
Product Citations: Sundararaj K, et al. 2021. Immunology. 162:418. PubMed
RRID: AB_2564117 (BioLegend Cat. No. 148601)
Structure: 89 kD type I transmembrane protein with 14 leucine-rich repeats (LRR) on its extracellular region and one toll/IL-1R (TIR) domain on its intracellular region.
Distribution: Monocytes, macrophages, dendritic cells, granulocytes, endothelial cells, epithelial cells.
Function: Activates innate host defense against a diverse set of pathogens.
Interaction: TLR1, TLR6.
Ligand/Receptor: Microbial lipoproteins, peptidoglycans, lipoteichoic acid.
Cell Type: Dendritic cells, Endothelial cells, Epithelial cells, Granulocytes, Macrophages, Monocytes
Biology Area: Cell Biology, Immunology, Innate Immunity, Signal Transduction
Molecular Family: CD Molecules, Toll Like Receptors
Antigen References: 1. Du B, et al. 2013. J. Biol. Chem. 288:15131. 2. Zivkovic A, et al. 2011. J. Immunol. 186:1608. 3. Okun E, et al. 2010. J. Neurochem. 114:462. 4. Kim S, et al. 2009. Nature. 457:102.
Gene ID: 24088
UniProt: View information about CD282 on UniProt.org
Clone: CB225
Regulatory Status: RUO
Other Names: TLR-2, mTLR2, Ly105
Isotype: Rat IgG2a, κ