Biolegend, 148605, Brilliant Violet 421™ anti-mouse CD282 (TLR2) Antibody, 50μg

CD282, also known as TLR2, is an 89 kD, type I transmembrane protein, with 14 leucine-rich repeats (LRR) in the extracellular region and one toll/IL-1R (TIR) domain in the intracellular region. TLR2 is mainly expressed on monocytes, macrophages, dendritic cells, granulocytes, and endothelial and epithelial cells. TLR2 associates with TLR1 and TLR6, and mediates the innate immune response against several components of Gram-positive bacteria, such as peptidoglycan, lipoteichoic acid (LTA), lipoarabinomanan, and lipoproteins as well as different LPS from certain Gram-negative bacteria, yeast, spirochetes, and fungi.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse TLR2-transfected Ba/F3 cells.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: 1. Shibata T, et al. 2012. Int. Immunol. 24:613. (FC)
RRID: AB_3106173 (BioLegend Cat. No. 148605)
Structure: 89 kD type I transmembrane protein with 14 leucine-rich repeats (LRR) on its extracellular region and one toll/IL-1R (TIR) domain on its intracellular region.
Distribution: Monocytes, macrophages, dendritic cells, granulocytes, endothelial cells, epithelial cells.
Function: Activates innate host defense against a diverse set of pathogens.
Interaction: TLR1, TLR6.
Ligand/Receptor: Microbial lipoproteins, peptidoglycans, lipoteichoic acid.
Cell Type: Dendritic cells, Endothelial cells, Epithelial cells, Granulocytes, Macrophages, Monocytes
Biology Area: Cell Biology, Immunology, Innate Immunity, Signal Transduction
Molecular Family: CD Molecules, Toll Like Receptors
Antigen References: 1. Du B, et al. 2013. J. Biol. Chem. 288:15131. 2. Zivkovic A, et al. 2011. J. Immunol. 186:1608. 3. Okun E, et al. 2010. J. Neurochem. 114:462. 4. Kim S, et al. 2009. Nature. 457:102.
Gene ID: 24088
UniProt: View information about CD282 on UniProt.org
Clone: CB225
Regulatory Status: RUO
Other Names: TLR-2, mTLR2, Ly105
Isotype: Rat IgG2a, κ
Q: What is the F/P ratio range of our BV421™ format antibody reagents?
A: It is lot-specific. On average it ranges between 2-4.