Biolegend, 148709, PerCP/Cyanine5.5 anti-mouse P2X7R Antibody, 25μg

P2X7R, also known as P2X7 receptor, belongs to the family of ligand-gated ion channel receptors. It is expressed on T cells, B cells, macrophages, and microglia. The receptor opens in the presence of extracellular ATP or NAD, leading to intracellular calcium mobilization. P2X7R activation requires higher concentrations of ATP compared to other P2X receptors. Longer stimulation results in larger pores, allowing passage of larger molecules. Activation of these molecules also leads to mitochondrial and cytoskeletal changes as well as IL-1β maturation and release. Ligation of P2X7 receptor can lead to membrane blebbing and cell death.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Murine colon mast cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications for the relevant formats include: immunoprecipitation1, Western blotting1, immunohistochemistry1, in vivo inhibition of intestinal inflammation and mast cell activation1.
Application References(PubMed link indicates BioLegend citation): Kurashima Y, et al. 2012. Nat. Commun. 3:1034. (FC, IP, WB, IHC, FA)
Product Citations: Lin YH 2023. Immunity. 56(1):207-223.e8. PubMed
RRID: AB_2728182 (BioLegend Cat. No. 148709) AB_2728183 (BioLegend Cat. No. 148710)
Structure: Ligand-gated ion channel, trimer - two transmembrane regions and one cytosolic region.
Distribution: Immune cells, including mast cells, microglia, macrophages, T cells, and B cells.
Function: Ion channel activated by extracellular ATP.
Ligand/Receptor: ATP, NAD.
Cell Type: B cells, Macrophages, Mast cells, T cells, Tregs
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Antigen References: 1. Surprenant A, et al. 1996. Science. 272:735. 2. Chused TM, et al. 1996. J. Immunol. 157:1371. 3. Gargett CE, et al. 1997. Br. J. Pharmacol. 122:911. 4. Kawamura H, et al. 2006. J. Immunol. 176:2152. 5. Pelegrin P. 2011. Br. J. Pharmacol. 163:908. 6. Bartlett R, et al. 2014. Pharmacol. Rev. 66:638. 7. Alves LA, et al. 2014. Biochim. Biophys. Acta. 1838:2578.
Gene ID: 18439
UniProt: View information about P2X7R on UniProt.org
Clone: 1F11
Regulatory Status: RUO
Other Names: P2X7 receptor (P2X7R), P2RX7
Isotype: Rat IgG2b, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.