Biolegend, 149207, Brilliant Violet 421™ anti-mouse ENPP1 Antibody, 50μg

ENPP1, also known as PC-1 or Ly-41, is a 115 kD type II transmembrane enzyme that is located on the cell's surface as a homodimer. ENPP1 hydrolyses ATP and other nucleoside 5' triphosphates to produce pyrophosphate which is an inhibitor of calcification and bone formation. It is involved in nucleotide recycling, cell adhesion, and regulates the signaling of insulin and purinergic receptors. ENPP1 is expressed by plasma cells, subsets of T and B1 cells, chondrocytes, capillary endothelium of the brain, renal distal tubules, and hepatocytes.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Murine T cell lymphoma EL4.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: This clone recognizes both alleles of ENPP1.Additional reported applications (for the relevant formats) include: Immunoprecipitation.2
Application References(PubMed link indicates BioLegend citation): Wang H, et al. 2012. Proc Natl Acad Sci USA 109:20077. (FC)Abbasi S, et al. 2011. Hybridoma 30:11. (FC, IP)Waterfield JD and Fairhurst M. 1991. Immunology 72:226. (FC)Takei F. 1984. J. Immunol. 4:1951. (FC, IP)
Product Citations: Wolock SL, et al. 2019. Cell Rep. 28:302. PubMed
RRID: AB_2565483 (BioLegend Cat. No. 149207)
Structure: Type II transmembrane protein, 115 kD, and forms homodimers.
Distribution: Plasma cells, a subset of T cells, a subset of B1 cells, chondrocytes, capillary endothelium of the brain, renal distal tubules, and hepatocytes.
Function: Generate pyrophosphate, recycle nucleotides, regulate cell adhesion, and to regulate signaling of insulin and purinergenic receptors.
Cell Type: Plasma cells, T cells, B cells
Biology Area: Cell Adhesion, Cell Biology, Immunology, Signal Transduction
Molecular Family: Phospho-Proteins
Antigen References: 1. Lau WM, et al. 2013. PLoS One. 8:e66752.2. Kato K, et al. 2012. Proc. Natl. Acad. Sci. USA 109:16876.3. Nam HK, et al. 2011. J Biol. Chem. 286:39059.4. Zhou HH, et al. 2009. Eur. J. Pharmacol. 616:346.
Gene ID: 18605
UniProt: View information about ENPP1 on UniProt.org
Clone: YE1/19.1
Regulatory Status: RUO
Other Names: Ectonucleotide pyrophosphatase/phosphodiesterase 1 (ENPP1), Ly-41, PC-1, NPP1, Plasma Cell Alloantigen 1 (PC1)
Isotype: Rat IgG2b, κ
Q: What is the F/P ratio range of our BV421™ format antibody reagents?
A: It is lot-specific. On average it ranges between 2-4.