Biolegend, 149403, PE anti-mouse CD130 (gp130) Antibody, 25μg

CD130, also known as gp130 and IL-6ST, is a type I transmembrane glycoprotein. CD130 can form receptor complexes for a family of cytokines, which include IL-6, IL-11, IL-27, LIF, and OSM, and can transduce signals for these cytokines. The 597aa extracellular domain has one Ig-like domain and five fibronectin type-III repeats. The 277a.a. intracellular domain does not have intrinsic kinase activity. Instead, it can be phosphorylated at tyrosine residues after it forms complexes with other receptor subunits, and in turn can lead to the association with JAK tyrosine kinases and the activation of STAT transcription factors, particularly STAT3.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant mouse CD130 (a.a. 23-619) expressed in 293E cells.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations: Huseni MA, et al. 2023. Cell Rep Med. 4:100878. PubMed Wight AE, et al. 2022. Proc Natl Acad Sci U S A. 119:e2200757119. PubMed van Oostrum M, et al. 2019. Nat Commun. 4.398611111. PubMed Ng S, et al. 2022. Sci Rep. 12:14087. PubMed
RRID: AB_2566644 (BioLegend Cat. No. 149403) AB_2566645 (BioLegend Cat. No. 149404)
Structure: Single pass type 1 membrane glycoprotein.
Distribution: T cells, activated B cells, plasma cells, and monocytes.
Function: Signal transduction.
Interaction: IL-6RA, IL-11RA, IL-27RA, OSMR, LIFR, CNTFR, JAK1, and SOCS3.
Cell Type: B cells, Dendritic cells, Monocytes, Plasma cells, T cells, Tregs
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules
Antigen References: 1. Hibi M, et al. 1990. Cell 63:1149. 2. Kishimoto T, et al. 1995. Blood 86:1243.
Gene ID: 16195
UniProt: View information about CD130 on UniProt.org
Clone: 4H1B35
Regulatory Status: RUO
Other Names: GP130, IL6ST, IL-6RB, IL-6R-beta, Oncostatin-M receptor subunit alpha, IL-6 receptor Beta
Isotype: Rat IgG2b, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.