Biolegend, 149520, PE/Dazzle™ 594 anti-mouse CD16.2 (FcγRIV) Antibody, 100μg

FcγRIV, also known as CD16.2, is an intermediate-affinity activating receptor for IgG2a and IgG2b. CD16.2 is the mouse homolog of human FcγRIIIA. CD16.2 is a low-affinity IgE receptor for all allotypes and the ligation of FcγRIV by antigen-IgE immune complexes promotes macrophage-mediated phagocytosis and is involved in lung inflammation.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Armenian Hamster
Immunogen: FCγR4 —EC domain fusion with IgG1 Fc
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats of this clone) include: blocking of Fc?RIV function1 and inhibition of immune complex binding1,2. The LEAF™ or Ultra-LEAF™ purified antibody (Endotoxin < EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (contact our custom solutions team).
Application References(PubMed link indicates BioLegend citation): Mancardi DA, et al. 2008. J. Clin. Invest 118:3738. (FC, Block) Nimmerjahn F, et al. 2005. Immunity 23:41.
RRID: AB_2632773 (BioLegend Cat. No. 149520)
Structure: 22.4 kD
Distribution: Expressed on peripheral blood leukocytes, spleen, colon, bone marrow, and the intestine
Function: Activating receptor for IgG2a and IgG2b via association with an ITAM bearing accessory.
Ligand/Receptor: IgG2a and IgG2b
Cell Type: B cells, Leukocytes
Biology Area: Immunology
Molecular Family: CD Molecules, Fc Receptors
Antigen References: Mechetina LV, et al. 2002. Immunogenetics. 54:463-8. Nimmerjahn F, et al. 2005. Immunity. 23:41-51. Seeling M, et al. 2013. Proc. Natl. Acad. Sci. 110:10729-34.
Gene ID: 246256
UniProt: View information about CD16.2 on UniProt.org
Clone: 9E9
Regulatory Status: RUO
Other Names: FcgRIV, Fcgr4, Fcrl3, CD16-2, FcgRIV, Fcgr3a, FcgammaRIV, 4833442P21Rik, Fc gamma RIV
Isotype: Armenian Hamster IgG