Biolegend, 149704, PE anti-mouse Granzyme A Antibody, 100μg

Granzyme A is a serine protease that is similar to Granzyme B. However, unlike granzyme B, granzyme A is a homodimer. It is expressed in cytotoxic T cells and natural killer cells (NK), but is not essential for induction of apoptosis or DNA fragmentation. Cell death induced by granzyme A is slower and morphologically different than the cell death induced by granzyme B. It plays a crucial role in infection-mediated inflammation as well as immunological responses against viruses, bacteria, and tumor cells.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Full length recombinant granzyme A of mouse origin.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References(PubMed link indicates BioLegend citation): Fehniger TA, et al. 2007. Immunity 26:798. (ICFC)
Product Citations: Corria-Osorio J, et al. 2023. Nat Immunol. 24:869. PubMed Saxena V, et al. 2022. Cell Rep. 39:110727. PubMed Santiago L, et al. 2020. Cell Rep. 107847:32. PubMed Findlay EG, et al. 2019. Oncoimmunology. 8:1608106. PubMed Wu J, et al. 2020. Sci Adv. 6:eaba3458. PubMed Jiang P, et al. 2021. Nat Methods. 18:1181. PubMed Uchil PD et al. 2018. Cell host & microbe. 25(1):87-100 . PubMed Wiedemann GM, et al. 2020. Cell Rep. 33:108498. PubMed Souza COS, et al. 2021. iScience. 24(6):102548. PubMed Thom SR, et al. 2017. J Appl Physiol (1985). 123:297. PubMed
RRID: AB_2565309 (BioLegend Cat. No. 149703) AB_2565310 (BioLegend Cat. No. 149704)
Structure: 29 kD, Homodimer, Serine protease
Distribution: Cytotoxic T cells and natural killer cells (NK); low on granulocytes, B cells, and activated dendritic cells (DCs).
Function: Induction of cell death and apoptosis.
Cell Type: T cells, NK cells
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroscience
Molecular Family: Proteases, Enzymes and Regulators
Antigen References: 1. Susanto O, et al. 2013. Cell Death Differ. 20:1183. 2. Ebnet K, et al. 1995. EMBO J. 14:4230. 3. Jenne DE, et al. 1988. Immunol. Rev. 103:53.
Gene ID: 14938
UniProt: View information about Granzyme A on UniProt.org
Clone: 3G8.5
Regulatory Status: RUO
Other Names: Hf, SE1, TSP1, Ctla3, TSP-1, Ctla-3, AW494114
Isotype: Mouse IgG2b, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.