Biolegend, 150342, Ultra-LEAF™ Purified anti-mouse CD198 (CCR8) Antibody, 1mg

C-C chemokine receptor type 8 (CCR8) CD198, is a 41 kD G-protein coupled receptor with 7 transmembrane regions. CCR8 is expressed by a subset of thymocytes, Tregs, NKT and Th2-polarized cells, a subset of macrophages, monocytes, and monocyte-derived dendritic cells. CCR8 mediates chemotaxis toward its ligand CCL1, and is involved in apoptosis of thymocytes.
1mg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse CD198 (CCR8)-transfected cells
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_3105975 (BioLegend Cat. No. 150341) AB_3105975 (BioLegend Cat. No. 150342)
Structure: G-protein coupled receptor, 7 transmembrane regions, 41 kD.
Distribution: Thymocytes, Tregs, NKT and Th2-polarized cells, subset of macrophages, monocytes and monocyte-derived dendritic cells.
Function: Chemotaxis, involved in thymocyte apoptosis
Ligand/Receptor: CCL1 (SCY1,TCA-3)
Cell Type: Dendritic cells, Macrophages, Monocytes, NKT cells, Th2, Thymocytes, Tregs
Biology Area: Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References: 1. Coghill JM, et al. 2013. Blood. 122:825. 2. Islam SA, et al. 2011. Nat. Immunol. 12:167. 3. Hoshino A, et al. 2007. J. Immunol. 178:5296. 3. Qu C, et al. 2004. J. Exp. Med. 200:1231.
Gene ID: 12776
UniProt: View information about CD198 on UniProt.org
Clone: SA214G2
Regulatory Status: RUO
Other Names: CDw198, C-C chemokine receptor type 8, CKR-8, CC-CKR-8, Ter1
Isotype: Rat IgG2b, κ
Q: Do you guarantee that your antibodies are totally pathogen free?
A: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Q: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
A: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Q: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
A: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.
Q: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
A: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.