Biolegend, 150604, Alexa Fluor® 647 anti-mouse CD192 (CCR2) Antibody, 100μg

CD192, also known as CCR2, is a 42 kD G-protein coupled receptor that is associated with bone marrow homeostasis. Specifically, CD192 mediates monocyte chemotaxis and acts as a receptor for monocyte chemoattractant protein 1 (MCP-1). CD192 is primarily expressed on monocytes and macrophages, with some expression on basophils. It is involved in monocyte infiltration in inflammatory diseases such as rheumatoid arthritis and cancer.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse CCR2 transfectants.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Product Citations: Christian DA, et al. 2022. Sci Immunol. 7:eabq7432. PubMed Mansouri N, et al. 2019. JCI Insight. 4:e128060. PubMed Sun G et al. 2018. Cell reports. 25(13):3786-3799 . PubMed Mariani SA, et al. 2019. Immunity. 50:1439. PubMed Linde N, et al. 2018. Nat Commun. 9:21. PubMed Oladiran O, et al. 2021. J Neuroinflammation. 77:18. PubMed Pascual–García M, et al. 2019. Nat Commun. 10:2416. PubMed Guo P, et al. 2021. J Immunol. 207:408. PubMed Blagih J, et al. 2020. Cell Rep. 30:481. PubMed Fan A, et al. 2020. J Neuroinflammation. 17:179. PubMed Wu B, et al. 2020. Int J Biol Sci. 1.132638889. PubMed Barbet G, et al. 2018. Immunity. 48:584. PubMed Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed Peruzzotti-Jametti L, et al. 2018. Cell Stem Cell. 22:355. PubMed Chen KE, et al. 2021. J Neuroinflammation. 18:140. PubMed Oladiran O, et al. 2021. Front Immunol. 12:720733. PubMed Kubli SP, et al. 2019. Nat Commun. 10:2678. PubMed van Ingen E, et al. 2021. Mol Ther Nucleic Acids. 26:1228. PubMed
RRID: AB_2566139 (BioLegend Cat. No. 150603) AB_2566140 (BioLegend Cat. No. 150604)
Structure: G-protein-coupled receptor, 7-transmembrane protein, 42 kD.
Distribution: Monocytes/macrophages and basophils.
Function: Mediator of monocyte chemotaxis and bone marrow homeostasis.
Ligand/Receptor: MCP-1, Ccl2, Ccl7, and Ccl13.
Cell Type: Basophils, Macrophages, Monocytes
Biology Area: Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References: 1. Mack M, et al. 2001. J. Immunol. 166:4697. 2. Dutta P, et al. 2015. Cell Stem Cell. 16:477. 3. Li L, et al. 2008. Kidney Int. 74:1526.
Gene ID: 12772
UniProt: View information about CD192 on UniProt.org
Clone: SA203G11
Regulatory Status: RUO
Other Names: Ccr2, Ckr2, Ccr2a, Ccr2b
Isotype: Rat IgG2b, κ