Biolegend, 150703, Alexa Fluor® 647 anti-mouse/human A2B5 Antibody, 25μg

The 105/A2B5 antibody recognizes a membrane protein that is expressed by chicken retinal cells, neuronal cells, ganglia, and spinal cord. In combination with other markers, this antibody can be used to differentiate between type 1 and type 2 astrocytes. The antibody recognizes polysialogangliosides and can be used to monitor glial cell development. A2B5 antigens are expressed by a variety of tumors including glioblastomas. Tumors expressing A2B5 antigen exhibit higher recurrence. The A2B5 antibody can also be used to identify progenitor cells.
25μg
Verified Reactivity: Mouse, Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Embryonic chicken retinal cells.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats of this clone) include: immunofluorescence staining.1,2
Application References(PubMed link indicates BioLegend citation): Eisenbarth GS, et al. 1979. Proc. Natl. Acad. Sci. 76:4913. (IF)Eisenbarth GS, et al. 1982. Proc. Natl. Acad. Sci. 79:5066. (IF)Russell TR, et al. 1984. Cytometry 5:539. (FC)
Product Citations: Freitag T, et al. 2024. NPJ Precis Oncol. 156:8. PubMed
RRID: AB_2566394 (BioLegend Cat. No. 150703) AB_2566395 (BioLegend Cat. No. 150704)
Distribution: Developing thymic epithelial cells, oligodendrocyte progenitors, neuroendocrine, embryonic neonatal tissue, astrocytoma, glioma, and squamous cell carcinoma.
Cell Type: Epithelial cells, Oligodendrocytes
Biology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Antigen References: 1. Baracskay KL, et al. 2007. GLIA 55:1001.2. Raff MC, et al. 1983. J. Neurosci. 6:1289.3. Fredman P, et al. 1984. Arch. Biochem. Biophys. 2:661.4. Xia CL, et al. 2003. Int. J. Oncol. 23:353.5. Tchoghandjian A, et al. Brain Pathol. 20:211.
Gene ID: NA
UniProt: View information about A2B5 on UniProt.org
Clone: 105
Regulatory Status: RUO
Other Names: F12A2B5, 105
Isotype: Mouse IgM, κ