Biolegend, 151206, Alexa Fluor® 647 anti-mouse/human Ki-67 Antibody, 100μg

The nuclear protein Ki-67 was first identified by the monoclonal antibody Ki-67, which was generated by immunizing mice with nuclei of the L428 Hodgkin lymphoma cell line. Ki-67 protein plays an essential role in ribosomal RNA transcription and cell proliferation. Expression of Ki-67 occurs during G1, S, G2, and M phase. While in G0 phase, the Ki-67 protein is not detectable. Ki-67 is strongly expressed in proliferating cells and has been reported as a prognostic marker in various tumors.
100μg
Verified Reactivity: Mouse, Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: E. coli expressed, N-terminal His-Thioredoxin-tagged, partial mKi-67 (1816-2163 aa) recombinant protein.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested ICC, IHC-F - Verified
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For intracellular flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. For immunocytochemistry, a concentration range of 2.5 - 5.0 μg/ml is recommended.For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 1.25 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Product Citations: Kobayashi T, et al. 2019. Cell. 176:982. PubMed Yang Y, et al. 2021. Nat Commun. 12:525. PubMed Sakamoto K, et al. 2021. Immunity. 54:2321. PubMed
RRID: AB_2566801 (BioLegend Cat. No. 151206)
Structure: 325 kD protein containing a forkhead-associated (FHA) domain and 13 tandem repeats.
Distribution: Nucleus and chromosomes.
Function: Required for cell cycle progression and proliferation.
Biology Area: Cell Biology, Cell Cycle/DNA Replication
Antigen References: Starborg M, et al. 1996. J. Cell. Sci. 109:143. Byeon IJ, et al. 2005. Nat. Struct. Mol. Biol. 12:987. Yerushalmi R, et al. 2010. Lancet. Oncol. 11:174. Beltrami AP, et al. 2001. N. Engl. J. Med. 344:1750. Sachsenberg N, et al. 1998. J. Exp. Med. 187:1295. Nagy Z, et al. 1997. Acta. Neuropathol. 93:294.
Gene ID: 428817345
UniProt: View information about Ki-67 on UniProt.org
Clone: 11F6
Regulatory Status: RUO
Other Names: Mki67, Ki67, Ki-67, MIB-1, KIA
Isotype: Rat IgG2b, κ