Biolegend, 151210, PE anti-mouse/human Ki-67 Antibody, 100μg

The nuclear protein Ki-67 was first identified by the monoclonal antibody Ki-67, which was generated by immunizing mice with nuclei of the L428 Hodgkin lymphoma cell line. Ki-67 protein plays an essential role in ribosomal RNA transcription and cell proliferation. Expression of Ki-67 occurs during G1, S, G2, and M phase. While in G0 phase, the Ki-67 protein is not detectable. Ki-67 is strongly expressed in proliferating cells and has been reported as a prognostic marker in various tumors.
100μg
Verified Reactivity: Mouse, Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: E. coli expressed, N-terminal His-Thioredoxin-tagged, partial mKi-67 (1816-2163 aa) recombinant protein.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations: Lao L, et al. 2023. Cancer Immunol Res. 11:320. PubMed Schwarz A, et al. 2023. Front Immunol. 14:1038689. PubMed Abe I, et al. 2022. Dev Cell. 57:2623. PubMed Xiao J, et al. 2023. J Clin Invest. 133: . PubMed Wight AE, et al. 2022. Proc Natl Acad Sci U S A. 119:e2200757119. PubMed Mogilenko DA, et al. 2020. Immunity. 54(1):99-115.e12. PubMed Low MSY, et al. 2019. Cell Reports. 29(9):2634-2645.e5. PubMed Lin J, et al. 2021. Front Immunol. 12:796260. PubMed Leonard NA, et al. 2021. Cancers (Basel). 13:. PubMed Brigas HC, et al. 2021. Cell Reports. 36(9):109574. PubMed Hasmim M, et al. 2022. Front Immunol. 13:982821. PubMed Oguri Y, et al. 2020. Cell. 182(3):563-577.e20. PubMed Zotos D, et al. 2021. Nat Commun. 12:7160. PubMed Zhao D, et al. 2020. Cancer Discov. 10:1374. PubMed Mansouri S, et al. 2020. Mucosal Immunol. 0.954861111. PubMed Wang H, et al. 2019. Stem Cells Int. 2019:4686132. PubMed Shan L, et al. 2017. Immunity. 47:766. PubMed
RRID: AB_2716014 (BioLegend Cat. No. 151209) AB_2716008 (BioLegend Cat. No. 151210)
Structure: 325 kD protein containing a forkhead-associated (FHA) domain and 13 tandem repeats.
Distribution: Nucleus and chromosomes.
Function: Required for cell cycle progression and proliferation.
Biology Area: Cell Biology, Cell Cycle/DNA Replication
Antigen References: Starborg M, et al. 1996. J. Cell. Sci. 109:143. Byeon IJ, et al. 2005. Nat. Struct. Mol. Biol. 12:987. Yerushalmi R, et al. 2010. Lancet. Oncol. 11:174. Beltrami AP, et al. 2001. N. Engl. J. Med. 344:1750. Sachsenberg N, et al. 1998. J. Exp. Med. 187:1295. Nagy Z, et al. 1997. Acta. Neuropathol. 93:294.
Gene ID: 428817345
UniProt: View information about Ki-67 on UniProt.org
Clone: 11F6
Regulatory Status: RUO
Other Names: Mki67, Ki67, Ki-67, MIB-1, KIA
Isotype: Rat IgG2b, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.