Biolegend, 151212, FITC anti-mouse/human Ki-67 Antibody, 100μg

The nuclear protein Ki-67 was first identified by the monoclonal antibody Ki-67, which was generated by immunizing mice with nuclei of the L428 Hodgkin lymphoma cell line. Ki-67 protein plays an essential role in ribosomal RNA transcription and cell proliferation. Expression of Ki-67 occurs during G1, S, G2, and M phase. While in G0 phase, the Ki-67 protein is not detectable. Ki-67 is strongly expressed in proliferating cells and has been reported as a prognostic marker in various tumors.
100μg
Verified Reactivity: Mouse, Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: E. coli expressed, N-terminal His-Thioredoxin-tagged, partial mKi-67 (1816-2163 aa) recombinant protein.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
Product Citations: Shiroshita K, et al. 2022. Cell Rep Methods. 2:100354. PubMed Elbasiony E, et al. 2023. NPJ Aging. 9:2. PubMed Pioli KT, et al. 2023. iScience. 26:106223. PubMed Huang F, et al. 2023. Int J Mol Sci. 24:. PubMed Lin G, et al. 2022. Int J Immunopathol Pharmacol. 36:3946320221117933. PubMed Meng L, et al. 2021. Front Oncol. 11:691705. PubMed Zhou WJ, et al. 2021. Theranostics. 11:5330. PubMed Snyder LM, et al. 2022. Immunohorizons. 6:660. PubMed Yang R, et al. 2020. Cell. 183(7):1826-1847.e31. PubMed Silconi ZB, et al. 2022. Int J Mol Sci. 23:. PubMed Yamada D, et al. 2022. Front Immunol. 13:1025499. PubMed Su X, et al. 2022. J Transl Med. 20:378. PubMed Xu P, et al. 2020. Cancer Immunol Res. 8:1193. PubMed Li X, et al. 2021. Front Cell Dev Biol. 9:647713. PubMed
RRID: AB_2814054 (BioLegend Cat. No. 151211) AB_2814055 (BioLegend Cat. No. 151212)
Structure: 325 kD protein containing a forkhead-associated (FHA) domain and 13 tandem repeats.
Distribution: Nucleus and chromosomes.
Function: Required for cell cycle progression and proliferation.
Biology Area: Cell Biology, Cell Cycle/DNA Replication
Antigen References: Starborg M, et al. 1996. J. Cell. Sci. 109:143. Byeon IJ, et al. 2005. Nat. Struct. Mol. Biol. 12:987. Yerushalmi R, et al. 2010. Lancet. Oncol. 11:174. Beltrami AP, et al. 2001. N. Engl. J. Med. 344:1750. Sachsenberg N, et al. 1998. J. Exp. Med. 187:1295. Nagy Z, et al. 1997. Acta. Neuropathol. 93:294.
Gene ID: 428817345
UniProt: View information about Ki-67 on UniProt.org
Clone: 11F6
Regulatory Status: RUO
Other Names: Mki67, Ki67, Ki-67, MIB-1, KIA
Isotype: Rat IgG2b, κ