Biolegend, 151702, Purified anti-mouse CD365 (Tim-1) Antibody, 100μg

CD365 (Tim-1) is a transmembrane protein also known as T cell immunoglobulin and mucin domain containing protein-1 and hepatitis virus cellular receptor 1. It is developmentally expressed at high levels in the blastocyst. Tim-1 is expressed on activated CD4 + lymphocytes especially on Th2 cells and has been implicated to play a critical role in the development of atopic disease and other Th2-biased immune responses. Tim-1 is hepatitis A virus receptor in humans. Tim-4 is the endogenous ligand of Tim-1. The interaction of Tim-1 and Tim-4 is involved in costimulation of T cell proliferation. Tim-1 is an endogenous ligand for LMIR5/CD300b.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant mouse TIM-1 (igV domain) FC chimera IFA
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality testedDirect ELISA - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.75 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Product Citations: Fonseca JA, et al. 2016. PLoS One. e0154819:11. PubMed Wagner AK, et al. 2017. Nat Commun. 8:15627. PubMed
RRID: AB_2616785 (BioLegend Cat. No. 151702)
Structure: Transmembrane protein containing immunoglobulin domain and mucin-like domain with the predicted molecular weight of 33 kD.
Distribution: Developmentally regulated; highly expressed in embryonic development in blastocysts. Expressed on activated CD4+ lymphocytes, especially Th2 cell lines.
Function: Binds hepatitis virus, may play a critical role in immune responses and the development of atopic diseases (in particular airway hyperreactivity).
Ligand/Receptor: Binds hepatitis A virus in humans, Tim-4, and LMIR5/CD300b.
Biology Area: Cell Biology, Cell Proliferation and Viability, Immunology
Molecular Family: CD Molecules, TCRs
Antigen References: 1. McIntire JJ, et al. 2001. Nat. Immunol. 2:1109. 2. Kuchroo VK, et al. 2003. Nat. Rev. Immunol. 3:454. 3. Wills-Karp M, et al. 2001. Nat. Rev. Immunol. 1:69. 4. Meyers JH, et al. 2005. Nat. Immunol. 6(5):455. 5. Umetsu S, et al. 2005. Nat. Immunol. 6:447. 6. Lee HH, et al. 2010. J. Immunol. 185:5225.
Gene ID: 171283
UniProt: View information about CD365 on UniProt.org
Clone: 3B3
Regulatory Status: RUO
Other Names: T cell immunoglobulin and mucin domain containing protein-1, T cell and airway phenotype regulator (Tapr), hepatitic virus cellular receptor 1, CD365
Isotype: Rat IgG2a, κ