Biolegend, 152104, Ultra-LEAF™ Purified anti-mouse CD20 Antibody, 1mg

CD20 is a 33-37 kD protein, a member of the MS4A family, with four transmembrane spanning regions that present as a homo-oligomeric complexes in the cell surface when associating with MHC class I and II, CD53, CD81, and CD82. CD20 is expressed on B cells and a subset of T cells, but not on plasma cells. CD20 regulates B-cell activation and proliferation. Its ligation promotes transmembrane Ca 2+ trafficking. CD20 is an important therapeutic target in the treatment of B cell lymphomas and leukemias.
1mg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse CD20 transfected cells
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: FC - Quality tested Depletion - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Clone SA271G2 has been validated for in vivo depletion of mouse B cells. Learn more...
Additional Product Notes: In rare cases, repeated administration of anti-mouse CD20 antibody, clone SA271G2 has resulted in sudden death of some animals. This reaction was not observed using anti-mouse CD20 antibody, clone QA18A73, which is a recombinant version of clone SA271G2, expressing a mouse isotype. View more applications data for this product in our Scientific Poster Library. Watch a Scientific Poster video of this product.
Application References(PubMed link indicates BioLegend citation): Bongard, A, et al. 2019. PLoS Pathog. 15(9):e1008043. (Depletion) Pubmed
Product Citations: Tang KH, et al. 2022. Cancer Discov. 12:47. PubMed Agrafiotis A, et al. 2023. Acta Neuropathol. 145:335. PubMed Carnasciali A, et al. 2021. J Neuroimmune Pharmacol. 16:376. PubMed Suah AN, et al. 2021. J Clin Invest. 131:. PubMed Sodir NM, et al. 2020. Cancer Discov. 0.825. PubMed Steffen E, et al. 2020. Basic Res Cardiol. 18:115. PubMed Vito A, et al. 2021. Commun Biol. 4:859. PubMed Kong H, et al. 2020. PLoS Pathog. 16:e1008947. PubMed Rocha-Resende C, et al. 2021. J Mol Cell Cardiol. 157:98. PubMed Hollern DP, et al. 2020. Cell. 179(5):1191-1206.e21.. PubMed Chen YQ, et al. 2019. J Immunother Cancer. 0.415277778. PubMed
RRID: AB_2629619 (BioLegend Cat. No. 152104) AB_2629619 (BioLegend Cat. No. 152115) AB_2629619 (BioLegend Cat. No. 152116)
Structure: Member of the MS4A family, four transmembrane spanning regions, three isoforms of 37, 35, and 37 kD, depending on the phosphorylation degree.
Distribution: B cells and a subset of T cells
Function: Regulates B-cell activation and proliferation
Interaction: Associates with MHC class I and II, CD53, CD81, and CD82
Cell Type: B cells, T cells
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Morsy DE, et al. 2013. J. Immunol. 191:3112. 2. Lund FE, Randall TD. 2010. Nat. Rev. Immunol. 10:236. 3.Beers SA, et al. 2010. Blood 115:5191. 4. Kuijpers TW, et al. 2010. J. Clin. Invest. 120:214.
Gene ID: 12482
UniProt: View information about CD20 on UniProt.org
Clone: SA271G2
Regulatory Status: RUO
Other Names: Ms4a1, Ly-44
Isotype: Rat IgG2b, κ
Q: Do you guarantee that your antibodies are totally pathogen free?
A: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Q: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
A: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Q: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
A: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.
Q: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
A: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.