Biolegend, 152210, PE/Dazzle™ 594 anti-mouse CD34 Antibody, 100μg

CD34, also known as gp105-120, is a highly glycosylated type I monomeric sialomucin-like glycophophoprotein with an approximate molecular weight of 105-120 kD, with two generated by alternative splicing. CD34 is expressed on hematopoietic progenitors, endothelial cells, brain, and testis. CD34 mediates cell adhesion and lymphocytes homing through binding to L-selectin and E-selectin ligands, and mediates the attachment of stem cells to the extracellular matrix or stromal cells. CD34 is phosphorylated on serine residues by PKC.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse CD34 transfected cells.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: This antibody (clone SA376A4) is useful for staining mouse CD34+ hematopoietic stem cell but it does not stain NIH-3T3 cells and the staining of Bend.3 cells is dimmer compared to other mouse CD34 antibodies, probably due to this antibody recognizes a different epitope than the other monoclonal antibodies.
RRID: AB_2734218 (BioLegend Cat. No. 152209) AB_2734219 (BioLegend Cat. No. 152210)
Structure: Type I monomeric glycophophoprotein with an approximate molecular weight of 105-120 kD.
Distribution: Hematopoietic progenitors, endothelial cells, brain and testis tissues.
Function: Cell adhesion and homing.
Interaction: PKC.
Ligand/Receptor: L-selectin and E-selectin.
Cell Type: Endothelial cells, Hematopoietic stem and progenitors
Biology Area: Cell Adhesion, Cell Biology, Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Garlanda C, et al. 1997. Eur J Cell Biol. 73:368-77. 2. Brown J, et al. 1991. Int Immunol. 3:175. 3. Suda J, et al. 1992. Blood. 79:2288-95. 4. Baumhueter S, et al. 1994. Blood. 84:2554.
Gene ID: 12490
UniProt: View information about CD34 on UniProt.org
Clone: SA376A4
Regulatory Status: RUO
Other Names: My10, gp105-120, mucosialin
Isotype: Rat IgG2a, λ