Biolegend, 152321, Alexa Fluor® 488 anti-mouse CD3ε Antibody, 25μg

CD3ε is a 20 kD transmembrane protein, also known as CD3 or T3. It is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3ε forms a TCR complex by associating with the CD3δ, γ and ζ chains, as well as the TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Syrian Hamster
Immunogen: Mouse T cell receptor.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested IHC-F, 3D IHC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. For immunohistochemistry on frozen tissue sections, a concentration range of 5.0 - 10 µg/ml is suggested. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Blue Laser (488 nm)
Application Notes: Clone 500A2 antibody is able to partially cross-block the 17A2 and 145-2C11 antibodies, indicating that all three of these anti-CD3 antibodies recognize distinct but overlapping epitopes.
Application References(PubMed link indicates BioLegend citation): Miescher GC, et al. 1989. Immunol. Lett. 23:113.
RRID: AB_2783123 (BioLegend Cat. No. 152321) AB_2783124 (BioLegend Cat. No. 152322)
Structure: Ig superfamily, forms CD3/TCR complex with CD3δ, γ and ζ subunits and TCR (α/β and γ/δ), 20 kD.
Distribution: Thymocytes (differentiation dependent), mature T cells and NK-T cells.
Function: TCR signal transduction, T cell activation and antigen recognition.
Interaction: T-cell receptor (TCR).
Ligand/Receptor: Peptide antigen/MHC-complex.
Cell Type: NKT cells, T cells, Thymocytes, Tregs
Biology Area: Cell Biology, Immunology, Signal Transduction
Molecular Family: CD Molecules, TCRs
Antigen References: 1. Barclay A, et al. 1997. The Leucocyte Antigen FactsBook Academic Press. 2. Davis MM. 1990. Annu. Rev. Biochem. 59:475. 3. Weiss A, et al. 1994. Cell. 76:263.
Gene ID: 12501
UniProt: View information about CD3epsilon on UniProt.org
Clone: 500A2
Regulatory Status: RUO
Other Names: CD3ε, T3, CD3
Isotype: Syrian Hamster IgG