Biolegend, 152412, APC/Cyanine7 anti-mouse CD19 Antibody, 100μg

CD19 is a 95 kD glycoprotein also known as B4. It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19. CD19, in association with CD21 and CD81, forms a molecular complex integral to B cell activation.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Transfected k562 cells expressing the extracellular domain of murine CD19.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with APC/Cyanine7 under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: blocking1,2 and immunoprecipitation1.
Application References(PubMed link indicates BioLegend citation): Krop I, et al. 1996. Eur. J. Immunol. 26:238. (FC, Blocking, IP) Abraham P, et al. 2014. Clin. Exp. Immunol. 175:181. (Blocking)
Product Citations: Zheng Y, et al. 2023. J Neuroimmune Pharmacol. . PubMed Sun Z, et al. 2023. Blood Sci. 160:5. PubMed Wang F, et al. 2024. Nat Commun. 203:15. PubMed
RRID: AB_2922473 (BioLegend Cat. No. 152411) AB_2922473 (BioLegend Cat. No. 152412)
Structure: Ig superfamily, associates with CD21 and CD81, 95 kD.
Distribution: Pro-B cells to mature B cells (during development), follicular dendritic cells.
Function: Modulates B cell activation and differentiation.
Ligand/Receptor: CD21, CD81, Leu-13.
Cell Type: B cells, Dendritic cells
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341. 2. Krop I, et al. 1996. Eur. J. Immunol. 26:238. 3. Krop I, et al. 1996. J. Immunol. 157:48. 4. Tedder TF, et al. 1994. Immunol. Today 15:437.
Gene ID: 12478
UniProt: View information about CD19 on UniProt.org
Clone: 1D3/CD19
Regulatory Status: RUO
Other Names: B4, CVID3
Isotype: Rat IgG2a, κ