Biolegend, 152602, Purified anti-mouse CD95 (Fas) Antibody, 500μg

CD95, also known as Fas, is an approximately 45 kD type I transmembrane protein belonging to the TNFR superfamily (TNFRSF6). The expression of CD95 has been described in various organs, such as  thymus, spleen, liver, heart, lung and ovary. Upon ligand (FasL) binding, CD95 forms the death-inducing signaling complex (DISC) intracellularly and induce apoptosis of the cell. CD95-induced apoptosis plays important roles in development, as well as in maintining peripheral tolerance of the immune system.
500μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Mouse Fas Transfectants
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Product Citations: Kobayashi A, et al. 2021. Front Immunol. 12:650856. PubMed Son YM, et al. 2020. Eur J Immunol. 50:1067. PubMed Mouat IC, et al. 2021. Elife. 10:. PubMed Wang J et al. 2018. Cell. 176(1-2):334-347 . PubMed Gubin MM, et al. 2018. Cell. 175:1014. PubMed Maier B, et al. 2020. Nature. 580:257. PubMed
RRID: AB_2629777 (BioLegend Cat. No. 152602)
Structure: CD95 (Fas) is an approximately 45 kD type I transmembrane protein; cystiene-rich domain, death domain.
Distribution: Thymus, spleen, liver, heart, lung, and ovary.
Function: Induction of apoptosis.
Interaction: Caspase 8, caspase 10, CFLAR, FADD, PDCD6, SUMO1, and TNFRSF6.
Ligand/Receptor: FasL
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology
Molecular Family: CD Molecules
Antigen References: Ogasawara J, et al. 1995. J Exp Med. 181:485-91. Ogasawara J, et al. 1993. Nature. 364:806-9. Nishimura Y, et al. 1995. J Immunol. 154:4395-403.
Gene ID: 14102
UniProt: View information about CD95 on UniProt.org
Clone: SA367H8
Regulatory Status: RUO
Other Names: Fas, ALPS1A, APO-1, APT1
Isotype: Mouse IgG1, κ