Biolegend, 153402, Purified anti-mouse CD125 (IL-5Rα) Antibody, 500μg

CD125 is a 60 kD type I transmembrane protein. It is the ligand-specific α subunit of the heterodimeric interleukin 5 (IL-5) receptor. The signal-transducing β subunit of this receptor does not bind to any cytokines and is shared by the receptors for IL-3 and CSF2/GM-CSF. IL-5 is mainly produced by Th2 cells and elevated IL-5 levels are associated with allergic disorders. CD125 has also been found to interact with syndecan binding protein (SDCBP or syntenin), leading to the activation of the transcription factor SOX4. The mouse CD125 is expressed on eosinophils, basophils, and B cells.
500μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Murine IL-5Ralpha-hFcgamma1 fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Product Citations: Wang Y, et al. 2021. Sci Transl Med. 13:. PubMed
RRID: AB_2715827 (BioLegend Cat. No. 153402)
Structure: 60 kD type I transmembrane protein
Distribution: Eosinophils, basophils and B cells
Function: IL-5 receptor
Interaction: JAK2, CD131 (IL3RB, CSF2RB), SDCBP
Ligand/Receptor: IL-5
Cell Type: B cells, Basophils, Eosinophils
Biology Area: Cell Biology, Cell Proliferation and Viability, Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Takaki S, et al. 1990. EMBO J. 9:4367 2. Takatsu K, et al. 2011. Proc. Jpn. Acad. Ser B. 87:463. 3. Hitoshi Y, et al. 1990. J. Immunol. 144:4218.
Gene ID: 16192
UniProt: View information about CD125 on UniProt.org
Clone: DIH37
Regulatory Status: RUO
Other Names: IL-5Rα, IL-5 Receptor α subunity
Isotype: Rat IgG2a, κ