Biolegend, 153503, PE anti-mouse CD215 (IL-15Rα) Antibody, 25μg

IL-15 receptor α (IL-15Rα) is a cytokine receptor with the structural homology to IL-2Rα (CD25). Both α receptors contain a group of β sandwich protein called sushi domains. Similar to IL-2Rα, IL-15Rα is able to form a heterotrimer with their β chain (CD122) and common γ chain (γc, CD132). But unlike IL-2Rα subunit, IL-15Rα itself binds to IL-15 with equivalent high affinity compared with the heterotrimeric complex. IL-15Rα is expressed as soluble or transmembrane forms by variety cells, such as activated monocytes, dendritic cells, CD8+ memory T cells, NK or NKT cells. IL-15Rα by itself forms stable complexes with IL-15 on cell surface and presents IL-15 in trans to neighboring cells. IL-15, like IL-2, is a pleiotropic four-helix bundle cytokine and plays a key role in promoting T and B cell survival and proliferation, preferentially in memory CD8 + T cell proliferation, and NK/NKT cell development.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse CD215 recombinant protein (33-205 a.a.)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations: Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed
RRID: AB_2721341 (BioLegend Cat. No. 153503) AB_2721342 (BioLegend Cat. No. 153504)
Structure: IL-15 receptor α (IL-15Rα) is a cytokine receptor with the structural homology of IL-2Rα (CD25). Both α receptors contain a group of β sandwich protein called sushi domains.
Distribution: It is found on a variety of subsets including dendritic cells, activated monocytes, memory CD8 T cells and NK/NKT cells.
Function: IL-15Rα is involved in T and B cell proliferation and development, preferentially for memory CD8+ T cell proliferation, and NK/NKT cell development
Ligand/Receptor: IL-15
Cell Type: Dendritic cells, Monocytes, NK cells, NKT cells, T cells
Biology Area: Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Giri JG, et al. 1995. EMBO J. 14:3654. 2. Giron-Michel J, et al. 2005. Blood. 106:2302. 3. Lorenzen I, et al. 2005. J. Biol. Chem. 281:6642. 4. Sato N, et al. 2007. Proc. Natl. Acad. Sci. USA. 104:588. 5. Anderson BG, Quinn LS. 2016. Endocrinology. 157:1315
Gene ID: 16169
UniProt: View information about CD215 on UniProt.org
Clone: 6B4C88
Regulatory Status: RUO
Other Names: IL-15RA, IL-15Rα, IL-15 Receptor α subunity
Isotype: Rat IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.