Biolegend, 153706, PE anti-mouse CX3CR1 Recombinant Antibody, 100μg

CX3CR1 is a 40 kD, G-protein coupled receptor, with seven transmembrane regions. CX3CR1 is expressed by resident and alternatively activated macrophages (M2), a subset of monocytes, dendritic cells (DCs), NK cells, a subset of memory T cells, and mast cells. CX3CR1 is involved in cell recruitment during inflammation and participates in cell adhesion and extravasation from blood vessels. Its ligand is CX3CL1, also known as fractalkine or neurotactin. CX3CR1 is also a coreceptor for HIV1 and variations in this gene leads to increased susceptibility to HIV. In the brain, it is expressed by glial cells, which interact with CX3CL1 expressed by neurons.
100μg
Verified Reactivity: Mouse
Antibody Type: Recombinant
Host Species: Mouse
Immunogen: Mouse CX3CR1-transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: This recombinant antibody clone is derived from clone SA011F11.
RRID: AB_2734221 (BioLegend Cat. No. 153705) AB_2734222 (BioLegend Cat. No. 153706)
Structure: G-protein coupled receptor, 7 transmembrane regions, 40 kD.
Distribution: Resident and alternatively activated macrophages (M2), subset of monocytes, dendritic cells (DCs), natural killer cells (NK), subset of memory T cells, mast cells, glial cells, and astrocytes.
Function: Involved in cell recruitment during inflammation, participates in cell adhesion and extravasation from blood vessels, coreceptor for HIV1.
Ligand/Receptor: CX3CL1 (fractalkine, neurotactin).
Cell Type: Astrocytes, Dendritic cells, Macrophages, Mast cells, Microglia, Monocytes, NK cells, T cells
Biology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience, Neuroscience Cell Markers
Molecular Family: Cytokine/Chemokine Receptors, GPCR
Antigen References: Hamon P, et al. 2017. Blood.129:1296 Afik R, et al. 2016. J Exp Med. 213:2315 Mass E, et al. 2016. Science. 353 pii: aaf4238 Safaiyan S, et al. 2016. Nat Neurosci. 19:995 Peng J, et al. 2016. Nat Commun. 7:12029
Gene ID: 13051
UniProt: View information about CX3CR1 on UniProt.org
Clone: QA16A03
Regulatory Status: RUO
Other Names: Chemokine (C-X3-C motif) receptor 1, Fractalkine receptor, GPR13, CCRL1, CMKBRL1, CMKDR1, V28
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.