Biolegend, 153806, PE anti-mouse CD184 (CXCR4) Recombinant Antibody, 100μg

CD184, also known as CXCR4, is a member of the G protein coupled receptor family that binds CXCL12 (SDF1). CXCR4 and CXCL12 play an important role in immune and inflammatory responses through the regulation of cell migration and growth. CXCR4 plays a crucial role in the pathogenesis of several autoimmune diseases such as atherosclerosis, rheumatoid arthritis, and wound healing. In addition, CXCR4 is the cofactor for fusion and entry of the T cell-tropic form of HIV-1.
100μg
Verified Reactivity: Mouse
Antibody Type: Recombinant
Host Species: Mouse
Immunogen: Mouse CXCR4-transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: This recombinant antibody clone is derived from clone L276F12.
Product Citations: Zhu L, et al. 2024. J Neuroinflammation. 112:21. PubMed Purvis GSD, et al. 2022. Br J Pharmacol. 179:2754. PubMed
RRID: AB_2734225 (BioLegend Cat. No. 153805) AB_2734226 (BioLegend Cat. No. 153806)
Structure: G-protein-coupled seven transmembrane receptor, 39.7 kD
Distribution: T lymphocytes, monocytes, macrophages, tissue-committed stem/progenitor cells (TCSCs), mast cells, vascular smooth muscle cells (VSMCs)
Function: Development of cardiovascular and central nervous system, hematopoiesis, and colonization of BM by fetal liver-derived hematopoietic stem cells (HSCs) during embryogenesis, homing and egress of CD34+ CXCR4+ progenitor cells from bone marrow, and their migration into peripheral tissues. Critical role in homing of cancer cells to specific metastatic sites. Possible role in the ubiquitin proteosome system.
Interaction: Robo -1
Ligand/Receptor: CXCL12 (SDF-1)
Cell Type: Macrophages, Mast cells, Monocytes, T cells
Biology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References: McDermott DH, et al. 2015. Cell. 160:686 Beck TC, et al. 2014. J Exp Med. 211:2567 Ding BS, et al. 2014. Nature. 505:97 Balabanian K, et al. 2012. Blood. 119:5722
Gene ID: 12767
UniProt: View information about CD184 on UniProt.org
Clone: QA16A08
Regulatory Status: RUO
Other Names: Fusin, LESTR, Sdf1r, Cmkar4, PB-CKR, PBSF/SDF-1
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.