Biolegend, 154306, PE anti-mouse Perforin Antibody, 100μg

Perforin is a 70 kD cytolytic protein that is expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. Perforin is one of the major effector molecules used by cytotoxic T cells and NK cells to mediate targeted cell lysis.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse perforin transfected cells.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Clone S16009A reacts with perforin from both C57BL/6 and BALB/c strains.
Product Citations: Wang M, et al. 2022. Adv Sci (Weinh). 9:e2202914. PubMed Pan Y, et al. 2023. Adv Sci (Weinh). 10:e2206792. PubMed Li H, et al. 2022. Cancer Cell. 40:36. PubMed Jia JJ, et al. 2022. J Leukoc Biol. 112:157. PubMed Campisi L, et al. 2022. Nature. 606:945. PubMed Liu L, et al. 2022. Nat Commun. 13:6740. PubMed Yang H, et al. 2023. Cell Death Differ. 30:560. PubMed Xu W 2023. Cell Reports. 42(1):111987. PubMed Wang W, et al. 2022. World J Gastrointest Oncol. 14:1124. PubMed Macchi C, et al. 2022. iScience. 25:104435. PubMed Reyes RM, et al. 2021. Oncoimmunology. 10:2006529. PubMed Lu Y, et al. 2020. Cell. 180(6):1081-1097. PubMed Jiang P, et al. 2021. Nat Methods. 18:1181. PubMed Goggi JL, et al. 2020. Mol Imaging Biol. 22:1392. PubMed Wu J, et al. 2020. Immunity. 53:115. PubMed Chandana M, et al. 2022. Nat Commun. 13:4028. PubMed Pathania AS, et al. 2022. Mol Ther Oncolytics. 25:308. PubMed Melo-Silva CR, et al. 2021. PLOS Pathogens. 17(5):e1009593. PubMed Lou Y, et al. 2021. Int J Mol Sci. 22:. PubMed Zhang X, et al. 2021. Nat Commun. 12:4536. PubMed Sade–Feldman M, et al. 2018. Cell. 175:998. PubMed Shen H, et al. 2022. Nat Commun. 13:5013. PubMed
RRID: AB_2721638 (BioLegend Cat. No. 154305) AB_2721639 (BioLegend Cat. No. 154306)
Structure: 70 kD Protein that oligomerizes for pore formation.
Distribution: CTL, NK (Cytoplasmic Granules)
Function: Target cell lysis
Cell Type: NK cells, T cells
Biology Area: Immunology
Molecular Family: Cytokines/Chemokines
Antigen References: Podack ER, et al. 1988. Immunol. Rev. 103:203. Konjar S, et al. 2010. Immunology. 131:257. Trapani JA, et al. 2002. Nat. Rev. Immunol. 2:735. Osinska I, et al. 2014. Cent. Eur. J. Immnol. 39:109.
Gene ID: 18646
UniProt: View information about Perforin on UniProt.org
Clone: S16009A
Regulatory Status: RUO
Other Names: Prf1, Pfp, Pfn, Pfn-1
Isotype: Rat IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.