Biolegend, 154508, PE/Cyanine7 anti-mouse β2-microglobulin Antibody, 100μg

Beta 2-microglobulin (β2M) is a 12 kDa, Ig like, non-membrane-anchored glycoprotein. It non-covalently associates with MHC class I heavy chain and some Class I-like molecules and serves as the light chain. In association with MHC class I, β2M is expressed on all leukocytes, platelets, endothelial cells, and epithelial cells. β2M plays an essential role in stabilizing MHC class I complexes for antigen presentation. In humans, circulating β2M levels are elevated in autoimmune/inflammatory disorders, viral infections, and glomerular filtration disorders.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant Mouse B2M
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations: Hum NR, et al. 2020. Cancers (Basel). :12. PubMed
RRID: AB_2728212 (BioLegend Cat. No. 154507) AB_2728213 (BioLegend Cat. No. 154508)
Structure: 12 kD, Ig like glycoprotein
Distribution: Leukocytes, platelets, endothelial cells, epithelial cells
Function: Component of MHC class I complex
Ligand/Receptor: TCR (through MHC class I)
Cell Type: Endothelial cells, Epithelial cells, Leukocytes, Platelets
Biology Area: Immunology
Molecular Family: MHC Antigens
Antigen References: Bernier GM, 1980, ox Sang, 38:323. Williams A, et al, 2002, Tissue Antigens, 59:3. Williams DB, et al, 1989, J. Immunol, 142:2796. Benoit LA, et al. 2007, J Immunol, 179:3588.
Gene ID: 12010
UniProt: View information about beta2-microglobulin on UniProt.org
Clone: A16041A
Regulatory Status: RUO
Other Names: Ly-m11, beta2m, beta2-m
Isotype: Rat IgG2a, κ