Biolegend, 154718, PE/Dazzle™ 594 anti-mouse CD204 Antibody, 100μg

CD204, also known as scavenger receptor A (SR-A) and the macrophage scavenger receptor (MSR), is 220 kDa, trimeric type II transmembrane protein, with one scavenger receptor cysteine-rich domain (SRCR). It is a phagocytic pattern-recognition receptor (PRR) expressed on macrophages and dendritic cells. CD204 is a receptor mediating recognition and internalization of low-density lipoprotein (LDL) by macrophages and plays a critical role in atherogenesis. CD204 also recognizes apoptotic cells, modified lipid proteins, and exogenous pathogen-associated molecular patterns (PAMPs), which results in the induction of innate immune and inflammatory responses. CD204 can act as a co-receptor for Toll-like receptors, such as TLR3, TLR4, or TLR9, to facilitate the expression of proinflammatory cytokines. CD204 has been implicated in several pathological processes such as Alzheimer’s disease, sepsis, ischemic injury, and coronary artery disease.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant mouse CD204 extracellular domain
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. * PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: This clone has minimal recognition of CD204 in C57BL/6.
RRID: AB_2892314 (BioLegend Cat. No. 154717) AB_2892314 (BioLegend Cat. No. 154718)
Structure: Trimeric type II transmembrane protein, one scavenger receptor cysteine-rich domain (SRCR), 220 kD.
Distribution: Macrophages, dendritic cells.
Function: Uptake of negatively charged molecules, role in pathology of atherosclerosis and Alzheimer’s disease.
Ligand/Receptor: Acetylated and oxidized lipoproteins
Cell Type: Dendritic cells, Macrophages
Antigen References: Shichita T, et al. 2017. Nat Med. 23:723. Muczynski V, et al. 2017. Blood. 129:2443. Iftakhar-E-Khuda I, et al. 2016. Proc Natl Acad Sci U S A. 113:10643. Nellimarla S, et al. 2015. J Immunol. 195:3858. Bonilla DL, et al. 2013. Immunity. 39:537.
Gene ID: 20288
UniProt: View information about CD204 on UniProt.org
Clone: 1F8C33
Regulatory Status: RUO
Other Names: Msr1, MSR, SR-A, Scavenger receptor type A, Macrophage scavenger receptor I, Scara1
Isotype: Rat IgG2a, κ