Biolegend, 155306, APC anti-mouse CD163 Antibody, 100μg

CD163 is a member of the group B scavenger receptor cysteine-rich superfamily, also known as GHI/61, M130, RM3/1, p155, hemoglobin-haptoglobin complex receptor, or macrophage-associated antigen. It is a 134 kD (non-reduced)/155 kD (reduced) glycoprotein primarily expressed on macrophages, Kupffer cells, monocytes, a subset of dendritic cells, and a subset of hematopoietic stem/progenitor cells. CD163 binds to haptoglobin-hemoglobin complex and TWEAK, and plays a role in clearing hemoglobin and regulating cytokine production by macrophages. Membrane CD163 can be cleaved by metalloproteinases (MMP), resulting in a soluble form. Elevated serum level of sCD163 has been implicated in many kinds of inflammatory diseases.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant mouse CD163 extracellular domain
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Product Citations: Lee CY, et al. 2023. Viruses. 15:. PubMed Poh AR, et al. 2022. Sci Adv. 8:eabl7882. PubMed Harb H, et al. 2021. Immunity. 54(6):1186-1199.e7. PubMed Jiang L, et al. 2020. Cell. 183(5):1219-1233.e18. PubMed Jiang H, et al. 2022. Cell Death Dis. 13:183. PubMed
RRID: AB_2814059 (BioLegend Cat. No. 155305) AB_2814060 (BioLegend Cat. No. 155306)
Structure: Type 1 transmembrane glycoprotein, 134 kD (non-reduced)/155 kD (reduced) glycoprotein
Distribution: Macrophages, Kupffer cells, monocytes, a subset of dendritic cells, and a subset of hematopoietic stem/progenitor cells
Function: Binds to haptoglobin-hemoglobin complex and TWEAK, and plays a role in clearing hemoglobin and regulating cytokine production by macrophages
Biology Area: Immunology
Molecular Family: CD Molecules
Gene ID: 93671
UniProt: View information about CD163 on UniProt.org
Clone: S15049I
Regulatory Status: RUO
Other Names: M130, MM130, SCARI1
Isotype: Rat IgG2a, κ