Biolegend, 155536, PerCP/Fire™ 806 anti-mouse CD170 (Siglec-F) Antibody, 100μg

CD170, also known as Siglec-F and Siglec-5, is a member of the sialic acid-binding immunoglobulin-like lectin (siglec) family of type I single pass transmembrane proteins.  Siglec-F has four extracellular Ig-like domains and two cytoplasmic ITIM motifs and preferentially binds [alpha]-2,3-linked sialic acid. Siglec-F is expressed on eosinophils, alveolar macrophages and intestinal microfold (M) cells and induces apoptosis of lung eosinophils during allergic asthma.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Fire™ 806 under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. * PerCP/Fire™ 806 has a maximum excitation of 478 nm and a maximum emission of 806 nm.
Excitation Laser: Blue Laser (488 nm)
RRID: AB_3068065 (BioLegend Cat. No. 155535) AB_3068065 (BioLegend Cat. No. 155536)
Structure: Member of the Sialic acid-binding Ig-like lectin family, type I, single pass, transmembrane protein, 4 extracellular Ig-like domains, 2 ITIM motifs in the cytoplasmic domain
Distribution: Eosinophils, alveolar macrophages, intestinal microfold (M) cells
Function: Induces apoptosis in eosinophils
Ligand/Receptor: [alpha]-2,3-linked sialic acid
Cell Type: Eosinophils, Macrophages
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Innate Immunity
Molecular Family: CD Molecules, Siglec Molecules
Antigen References: Gicheva N, et al. 2016. Biochem. Biophys. Res. Commun. 479:1. Kiwamoto T, et al. 2015. J. Allergy Clin. Immunol. 135:1329. Suzukawa M, et al. 2013. J. Immunol. 190:5939. Patnode ML, et al. 2013. J. Biol. Chem. 288:26533.
Gene ID: 233186
UniProt: View information about CD170 on UniProt.org
Clone: S17007L
Regulatory Status: RUO
Other Names: Siglec-5
Isotype: Rat IgG2a, κ