Biolegend, 156803, Alexa Fluor® 647 anti-mouse CLEC4F Antibody, 25μg

CLEC4F, also known as Kupffer cell receptor or KCR, is an 80 kDa type II transmembrane glycoprotein of the C-type lectin superfamily. It is expressed on resident Kupffer cells and infiltrating monocytes in the liver but not on macrophages in other tissues. CLEC4F interacts with alpha-galactosylceramide (α-GalCer) in a calcium-dependent manner and participates in the presentation of α-GalCer to natural killer T (NKT) cells.
25μg
Verified Reactivity: Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: IHC-F - Quality tested 3D IHC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 2.5 - 10 µg/mL is suggested. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Product Citations: Zeng J, et al. 2023. Nat Commun. 14:2573. PubMed Shang Y, et al. 2022. Br J Pharmacol. 179:4378. PubMed Chen WS, et al. 2021. Cell Rep. 37:109974. PubMed Glennon EKK, et al. 2022. Front Cell Infect Microbiol. 11:804186. PubMed Lam P, et al. 2022. Mol Ther Methods Clin Dev. 26:413. PubMed Cho CS, et al. 2021. Cell. 184(13):3559-3572.e22. PubMed
RRID: AB_2814081 (BioLegend Cat. No. 156803) AB_2814082 (BioLegend Cat. No. 156804)
Structure: Type II transmembrane glycoprotein
Distribution: Kupffer cells
Function: Modulates glycolipids presentation on Kupffer cells.
Ligand/Receptor: Fucose, Galactose, N-acetylgalactosamine, un-sialylated glucosphingolipids with GalNAc or Gal terminus
Antigen References: Yang CY, et al. 2013. PLoS One. 8(6):e65070. van der Tuin SJL, et al. 2018. J Am Heart Assoc. 7(6) Hsieh SL, et al. 2009. J. Immunol. 182:78.38.
Gene ID: 51811
UniProt: View information about CLEC4F on UniProt.org
Clone: 3E3F9
Regulatory Status: RUO
Other Names: C-Type Lectin Domain Family 4 Member F (CLEC4F), C-Type Lectin, Superfamily Member 13 (CLECSF13), Kupffer cell receptor (KCR), KCLR
Isotype: Mouse IgG1, κ