Biolegend, 157506, PE anti-mouse CD40 Antibody, 100μg

CD40 is a 48 kD type I transmembrane glycoprotein also known as Bp50. It is a member of the tumor necrosis factor receptor (TNFR) superfamily and is expressed on B cells, basal epithelial cells, macrophages, follicular dendritic cells, endothelial cells, and a subset of CD34 + hematopoietic progenitors. CD40 regulates B cell development/maturation, Ig isotype switching and, in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with its ligand CD154 (gp39), which is expressed on activated T cells, is important in costimulation and immune regulation.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant mouse CD40 – Fc fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: The FGK45 antibody can block the binding of HM40-3 clone. The FGK45 antibody can also stimulate mouse B cell proliferation.
Application References(PubMed link indicates BioLegend citation): Andersson J, et al. 1996. Immunity 5:319-330. Melief CJ, et al. 1998. Nature 393(6684):480-3. Londei M, et al. 2000. Nat Med 6(6):673-9. Tominaga T, et al. 2015. Neuro Oncol 17(11):1453-62.
Product Citations: Bhaskar A, et al. 2023. iScience. 26:106644. PubMed Wang R, et al. 2022. J Immunother Cancer. 10:. PubMed
RRID: AB_2832552 (BioLegend Cat. No. 157505) AB_2860731 (BioLegend Cat. No. 157506)
Structure: 48 kD type I transmembrane glycoprotein, a member of the tumor necrosis factor receptor (TNFR) superfamily.
Distribution: Expressed on B lymphocytes and other antigen-presenting cells, such as macrophages, follicular dendritic cells, etc.
Function: Regulates B cell growth and differentiation and Ig isotype switching. Interaction of CD40 with its ligand, CD154, on activated T cells is important for immune response.
Interaction: TRAF molecules
Ligand/Receptor: CD154 (CD40L)
Cell Type: Antigen-presenting cells, B cells, Dendritic cells, Macrophages
Biology Area: Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules
Antigen References: Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. Bancherou J, et al. 1994. Annu. Rev. Immunol. 12:881. Clark EA, et al. 1996. P. Natl. Acad. Sci. USA 83:4494. Grewal IS, et al. 1998. Annu Rev Immunol 16:111.
Gene ID: 21939
UniProt: View information about CD40 on UniProt.org
Clone: FGK45
Regulatory Status: RUO
Other Names: Bp50, TNFRSF5
Isotype: Rat IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.