Biolegend, 159503, PE anti-mouse PD-1H (VISTA) Antibody, 25μg

PD-1H, also known as VISTA, is a 309 aa type I transmembrane protein, composed of seven exons. PD-1H has one Ig-V like domain, and its sequence is similar to the Ig-V domains of the members of CD28 and B7 families. PD-1H is expressed by a subset of T cells, macrophages, dendritic cells, neutrophils, and NK cells. It has been proposed that PD-1H can be useful to modulate the host immune response to allogeneic transplants.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant mouse VISTA protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Clone A17207C antibody does not affect the binding of clone MIH63 nor clone MH5A.
RRID: AB_2890722 (BioLegend Cat. No. 159503) AB_2890722 (BioLegend Cat. No. 159504)
Structure: Type I transmembrane protein, member of the CD28 family
Distribution: Macrophages, dendritic cells, neutrophils, NK cells, naïve and activated T cells
Function: Negative regulation of immune response
Ligand/Receptor: PSGL-1
Cell Type: Dendritic cells, Macrophages, Neutrophils, NK cells, T cells, Tregs
Biology Area: Immunology, Inhibitory Molecules
Molecular Family: Soluble Receptors
Antigen References: Flies DB, et al. 2011. J. Immunol. 187:1537. Wang Li, et al. 2011. J. Exp Med. 208:577.
Gene ID: 74048
UniProt: View information about PD-1H on UniProt.org
Clone: A17207C
Regulatory Status: RUO
Other Names: PD-1 homolog, Dies1, B7-H5
Isotype: Rat IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.