Biolegend, 160402, Purified anti-mouse CD126 (IL-6Rα) Antibody, 500μg

CD126 (IL-6Rα) is an 80kD member of the immunoglobulin superfamily that is expressed on activated T and B cells, monocytes, hepatocytes, and plasma cells. High affinity IL-6 receptors are formed by the non-covalent association of CD126 and the IL-6 receptor β chain (CD130 or gp130). CD126 binds IL-6 with low affinity, but does not signal. The β chain (gp130, CD130) does not bind IL-6 by itself, but associates with the α-chain/IL-6 complex to initiate signal transduction. IL-6 binding to the receptor complex results in the stimulation of B and T cells, and hematopoietic precursor proliferation and differentiation.
500μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse IL-6R recombinant protein, 1-357 a.a.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested Block - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone W18166A binds to IL-6 receptor alpha, inhibits IL-6/IL-6 receptor interaction and mediated phosphorylation of signal transducer and activator of transcription 3 (STAT3). This clone does not cross-react with rat.
RRID: AB_2876570 (BioLegend Cat. No. 160402)
Structure: Ig superfamily, associates with IL-6Rβ chain (CD130, gp130), 80 kD
Distribution: T cells and activated B cells, monocytes, hepatocytes, plasma cells
Function: Stimulates T cells, B cells, hematopoietic precursor proliferation and differentiation
Ligand/Receptor: IL-6
Cell Type: B cells, Epithelial cells, Fibroblasts, Granulocytes, Monocytes, Plasma cells, T cells
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience, Signal Transduction
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: Taga T, et al. 1997. Annu Rev Immunol. 15:797-819. Fitzgerald K, et al. 2001. The Cytokine FactsBook. Academic Press London. Boulanger MJ, et al. 2003. Science. 300:2101-4
Gene ID: 16194
UniProt: View information about CD126 on UniProt.org
Clone: W18166A
Regulatory Status: RUO
Other Names: Interluekin 6 receptor alpha, CD126
Isotype: Rat IgG2a, κ