Biolegend, 160604, PE anti-mouse TCR Vγ4 Antibody, 100μg

T cell receptor (TCR) is a heterodimer consisting of an α and β chain (TCR α/β) or a γ and δ chain (TCR γ/δ). TCR associates with CD3 to form a CD3/TCR complex. The CD3/TCR plays a key role in antigen recognition, signal transduction, and T cell activation. γδ T cells (gamma delta T cells) are a small subset of T cells that express a unique TCR. They are found in the thymus, peripheral lymphoid tissue, and peritoneum. Unlike αβ T cells, γδ T cells do not require antigen processing in order to recognize certain bacterial, lipid, and tumor antigens. The 49.2 antibody reacts specifically with mouse TCR Vγ4 as designated by Heilig/Tonegawa nomenclature. 2 Mouse TCR Vγ4 is also known as TCR Vγ2 under the Garman nomenclature system. Several TCR γ and δ chain nomenclature systems exist. In order to consolidate the various nomenclature systems, we have created charts for corresponding names across some of the most popular naming methods. Click here to see charts .
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: C57BL/6 thymocytes expanded in vitro in anti-TCRγδ antibody coated plates
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References(PubMed link indicates BioLegend citation): Khairallah C, et al. 2015. PLoS Pathog. 11:e1004702. Perira P, et al. 2000. Eur J Immunol. 30:1988-97.
RRID: AB_3675011 (BioLegend Cat. No. 160603) AB_3675011 (BioLegend Cat. No. 160604)
Structure: Heterodimer with γ and δ chain
Distribution: Subset of γδ T cells
Function: Antigen recognition
Interaction: CD3
Cell Type: Lymphocytes, T cells, Thymocytes
Biology Area: Adaptive Immunity, Immunology, Innate Immunity
Molecular Family: TCRs
Gene ID: 21638
UniProt: View information about TCR Vgamma4 on UniProt.org
Clone: 49.2
Regulatory Status: RUO
Other Names: T Cell Receptor Vγ4, TCR Vγ2, TRGV4, GV3S1
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.