Biolegend, 160802, Ultra-LEAF™ Purified anti-mouse CD70 Antibody, 1mg

CD70, or CD27L, is a 50 kD type II transmembrane glycoprotein and member of the tumor necrosis factor superfamily. Mouse CD27L and its human counterpart share about 56% amino acid sequence identity. The interaction between CD27L and CD27, a protein of the TNF receptor superfamily expressed on NK cells and subsets of T and B cells, provides costimulatory signals that are required for T cell proliferation, clonal expansion, and promotion of effector T cell formation. CD27L-CD27 interaction on mouse B cells on the other hand, has been shown to inhibit terminal differentiation of B cells into plasma cells. CD27L expression is induced by antigen receptor activation in B cells and at low levels in mouse T cells. CD27 interaction with recombinant soluble CD27L protein (sCD27L), has shown that sCD27L enhances IFN-γ secretion by CD56 (bright) NK cells in the presence of IL-12, and these NK cells were able to suppress HCV replication. Also, CD8+ T cells stimulated with sCD27L in the presence of Ag showed enhanced proliferation and production of IL-2 and IFN-γ in vitro. In addition, administration of Ag and soluble CD27L induces expansion of Ag-specific CD8+ T cells in vivo. Data suggests that sCD27L is a potent stimulator of antiglioma immune responses that depend on CD8+ T cells; therefore, sCD27L might be an adjuvant for future immunotherapy trials for glioblastoma.
1mg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant mouse CD70
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: Block - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by blocking the binding between mouse CD27 (TNFRSF7) and mouse CD27L (CD70). The ND50 range: 0.3 – 1.5 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application
RRID: AB_2890724 (BioLegend Cat. No. 160801) AB_2890724 (BioLegend Cat. No. 160802)
Structure: Homodimer
Distribution: Activated T cells, B cells, NK cells, antigen presenting cells, activated plasmacytoid dendritic cells (pDCs), chronic B cell lymphocytic leukemia, and large B cell lymphomas.
Function: Role in T cell proliferation, generation of cytolytic and memory T cells, B cell proliferation and differentiation. CD27L is induced by antigen receptor activation in B cells and downregulated by interaction with its receptor CD27.
Interaction: T cells, B cells, NK cells.
Ligand/Receptor: CD27
Cell Type: B cells, NK cells, T cells, Thymocytes
Biology Area: Cancer Biomarkers, Cell Proliferation and Viability, Immunology
Molecular Family: Cytokines/Chemokines, Growth Factors, Immune Checkpoint Receptors, Soluble Receptors
Antigen References: Bowman MR, et al. 1994. J Immunol. 152:1756-61. Shaw J, et al. 2010. Blood. 115:3051-7. Keller AM, et al. 2009. Blood. 113:5167-75. Tesselaar K, et al. 1997. J Immunol. 159:4959-65. Akiba H, et al. 1999. J Immunol. 162:7058-66. Miller J, et al. 2010. J Neurosurg. 113:280-5. Kuka M, et al. 2013. J Immunol. 191:2282-9. Jang YS, et al. 2013. Clin Immunol. 149:379-87.
Gene ID: 21948
UniProt: View information about CD70 on UniProt.org
Clone: A19034C
Regulatory Status: RUO
Other Names: CD27 ligand, CD70, Ki-24 antigen, TNFSF7
Isotype: Rat IgG2a, κ
Q: Do you guarantee that your antibodies are totally pathogen free?
A: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Q: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
A: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Q: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
A: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.
Q: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
A: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.