Biolegend, 161007, FITC anti-mouse CD64 (FcγRI) Antibody, 25μg

CD64 is a 72 kD single chain type I glycoprotein also known as FcγRI and FcRI. CD64 is a member of the immunoglobulin superfamily and is expressed on monocytes/macrophages, dendritic cells, and mast cells. The expression can be upregulated by IFN-γ stimulation. CD64 binds IgG immune complex. It plays a role in antigen capture, phagocytosis of IgG/antigen complexes, and antibody-dependent cellular cytotoxicity (ADCC).
25μg
Verified Reactivity: Mouse, Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant mouse FcγRI/CD64 (Cat. No. 773806)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality Tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
Product Citations: Crossey E, et al. 2024. Sci Rep. 21324:14. PubMed
RRID: AB_2910337 (BioLegend Cat. No. 161007) AB_2910337 (BioLegend Cat. No. 161008)
Structure: Ig superfamily, type I glycoprotein, 72 kD
Distribution: Monocytes, macrophages, mast cells, dendritic cells
Function: Phagocytosis, ADCC
Ligand/Receptor: IgG
Antigen References: 1. Schyns J, et al. 2019. Nat. Commun. 3;10(1):3964 2. Langlet C, et al. 2012. J. Immunol. 188 (4) 1751-1760.
Gene ID: 14129
UniProt: View information about CD64 on UniProt.org
Clone: S18017D
Regulatory Status: RUO
Other Names: FcRI
Isotype: Rat IgG2a, κ