Biolegend, 161504, PE anti-mouse CD117 (c-kit) Antibody, 100μg

CD117 is a 145 kD immunoglobulin superfamily member also known as c-kit and stem cell factor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor). CD117 is expressed on hematopoietic stem cells (including multipotent hematopoietic stem cells, progenitors committed to myeloid and/or erythroid lineages, and T and B cell precursors), mast cells, and acute myeloid leukemia (AML) cells. CD117 interaction with its ligand is critical for the development of hematopoietic stem cells.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse c-kit transfectants
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: S18020A has been tested to block the ligand binding of SCF on c-Kit which is determined by the phosphorylation of ERK1/2 in c-Kit signaling.
RRID: AB_2894651 (BioLegend Cat. No. 161503) AB_2894651 (BioLegend Cat. No. 161504)
Structure: A 145 kD immunoglobulin superfamily member
Distribution: Hematopoietic stem cells, mast cells, and some acute myeloid leukemia cells
Function: The development of hepatopoietic stem cells
Ligand/Receptor: Steel factor, stem cell factor, and mast cell growth factor
Cell Type: Hematopoietic stem and progenitors, Mast cells
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook (2nd ed.). Academic Press. 2. Galli SJ, et al. 1994. Adv. Immunol. 55:1. 3. Ikuta K, et al. 1992. Annu. Rev. Immunol. 10:759. 4. Besmer P, et al. 1986. Nature 320:415. 5. Witte ON. 1990. Cell 63:5.
Gene ID: 16590
UniProt: View information about CD117 on UniProt.org
Clone: S18020A
Regulatory Status: RUO
Other Names: SCO1, SOW3, CD117, Tr-kitm, Gsfsco5, c-kit
Isotype: Rat IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.