Biolegend, 161703, APC anti-mouse TCR Vγ7 Antibody, 25μg

T cell receptor (TCR) is a heterodimer consisting of α/β chains (αβ T cells) or γ/δ chains (γδ T cells). γδ T cells are a minor T cell population in the body, with the usage of γ and δ often associated with specific anatomical localization. Vγ7-expressing γδ T cells are resident in the intestinal epithelium and contain a high proportion of extrathymically generated cells. It has been reported that butyrophilin-like molecules Btnl1 with Btnl6 are important in Vγ7 + T cell responses. The F2.67 antibody reacts specifically with mouse TCR Vγ7 (as referred to by the Tonegawa nomenclature system for TCR γ and δ chains). Mouse TCR Vγ7 is also known as TCR Vγ5 under the Garman nomenclature system. Several TCR γ and δ chain nomenclature systems exist. In order to consolidate the various nomenclature systems, we have created charts for corresponding names across some of the most popular naming methods. Click here to see charts .
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Mouse hybridoma expressing anti Vγ7Vδ5 TCR
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application References(PubMed link indicates BioLegend citation): Pereira P, et al. 2000. Eur J Immunol. 30:1988-97. Khairallah C, et al. 2015. PLoS Pathog. 11:e1004702. Di Marco Barros R, et al. 2016. Cell. 167:203-218.
RRID: AB_3683180 (BioLegend Cat. No. 161703) AB_3683180 (BioLegend Cat. No. 161704)
Structure: Heterodimer with γ and δ chain
Distribution: Subset of γδ T cells
Function: Antigen recognition
Interaction: CD3
Cell Type: Lymphocytes, T cells, Thymocytes
Biology Area: Adaptive Immunity, Immunology, Innate Immunity
Molecular Family: TCRs
Gene ID: 21641
UniProt: View information about TCR Vgamma7 on UniProt.org
Clone: F2.67
Regulatory Status: RUO
Other Names: TCR Vgamma7, TCR Vγ5 (Garman nomenclature), TRGV7, GV4S1
Isotype: Mouse IgG2a, κ