Biolegend, 162602, Purified anti-mouse CD150 (SLAM) Antibody, 100μg

CD150 is a 75-95 kD member of the immunoglobulin superfamily, also known as SLAM (signaling lymphocyte activation molecule) or IPO-3. CD150, a single chain type I transmembrane molecule, is expressed on thymocytes, T cell subsets, B cells, dendritic cells, and endothelial cells. The expression is upregulated upon activation. CD150 expression has been shown to be maintained on Th1 but not Th2 clones. T regulatory cells express a relatively high level of CD150. Antibodies against CD150 have been shown to augment IFN-γ production by Th1 cells, especially when co-stimulated through the TCR. CD150 associates with the src homology 2-domain-containing protein tyrosine phosphatase SHP-2, and this association is thought to be involved in signal transduction. In combination with CD48, CD150 is a useful marker for hematopoietic stem cell studies.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: RBL1 transfectant (1-287a.a)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2894667 (BioLegend Cat. No. 162602)
Structure: Ig superfamily, 75-95 kD
Distribution: Thymocytes, T cell subset, B lymphocytes, dendritic cells, endothelial cells
Function: B cell and dendritic cell costimulation
Ligand/Receptor: CD150
Antigen References: 1. Punnonen J, et al. 1997. J. Exp. Med. 185:993. 2. Gordiienko I, et al. 2019. Clin Immunol. 204:14-22 3. Kiel M, et al. 2005. Cell. 121(7):1109-21
Gene ID: 27218
UniProt: View information about CD150 on UniProt.org
Clone: W19132B
Regulatory Status: RUO
Other Names: SignalingLymphocyte Activation Molecule (SLAM), IPO-3
Isotype: Rat IgG2a, λ