Biolegend, 163507, PE/Cyanine7 anti-mouse IFN-γ Antibody, 25μg

IFN-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID: AB_3097421 (BioLegend Cat. No. 163507) AB_3097421 (BioLegend Cat. No. 163508)
Structure: Cytokine; dimer; 40-80 kD (Mammalian)
Distribution: Tregs
Function: Upregulated by IL-2, FGF-basic, EGF; downregulated by 1-α-25-Dihydroxy vitamin D3, dexamethasone
Ligand/Receptor: Receptors: IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1)
Antigen References: 1. Zaidi et al. 2011, Clin Cancer Res,17(19):6118-24 2. Castro et al. 2018, Front Immunol, 9:847 3. Alspach et al. 2019, Cold Spring Harb Perspect Biol, 11(3):a028480
Gene ID: 15978
UniProt: View information about IFN-gamma on UniProt.org
Clone: W18272D
Regulatory Status: RUO
Other Names: Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF)
Isotype: Rat IgG2b, κ