Biolegend, 164805, Spark PLUS UV395™ anti-mouse CD90.2 (Thy1.2) Antibody, 25μg

CD90.2 is a 25-35 kD immunoglobulin superfamily member also known as Thy1.2. It is expressed on hematopoietic stem cells and neurons, all thymocytes, and peripheral T cells in Thy1.2 bearing mouse strains (Balb/c, CBA/J, C3H/He, C57BL/-, DBA, NZB/-). CD90.2 is a glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein involved in signal transduction. CD90.2 is involved in costimulation of lymphocyte proliferation and induction of hematopoietic stem cells differentiation. CD90.2 has been shown to interact with CD45.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Mouse Thy1.2 transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with Spark PLUS UV395™ under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. * Spark PLUS UV395™ has a maximum excitation of 355 nm and a maximum emission of 385 nm.
Excitation Laser: Ultraviolet Laser (355 nm)
Application Notes: Clone S20008D antibody reacts with mouse CD90.2 (Thy 1.2) which is expressed by C57BL/6 and BALB/C strains, but not on CD90.1 strains (AKR/J, PL, FVB, etc.). Clone S20008D can completely block binding of clone 30-H12 on target cells. To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with TruStain FcX™ PLUS (anti-mouse CD16/32) Antibody (Cat. No. 156603 & 156604) is recommended prior to staining.
RRID: AB_3683183 (BioLegend Cat. No. 164805) AB_3683183 (BioLegend Cat. No. 164806)
Structure: Ig superfamily, 25-35 kD
Distribution: Hematopoietic stem cells and neurons, all thymocytes, peripheral T cells of the Thy-1.2 bearing mice
Function: Lymphocyte costimulation, proliferation and differentiation of hematopoietic stem cells
Ligand/Receptor: CD45
Cell Type: Antigen-presenting cells, B cells, Dendritic cells, Endothelial cells, Fibroblasts, Hematopoietic stem and progenitors, Mesenchymal Stem Cells, Neurons, T cells, Thymocytes
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Adhesion, Cell Death, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. Craig W, et al. 1993. J Exp Med. 177:1331-42. Reif AE & Schlesinger M. 1989. Cell Surface Antigen Thy-1. Mayani H, et al. 1994. Blood. 83:2410-7.
Gene ID: 21838
UniProt: View information about CD90.2 on UniProt.org
Clone: S20008D
Regulatory Status: RUO
Other Names: Thy-1, Thy-1.2, CD90, CD90.2, Th1.2
Isotype: Mouse IgG2b, κ