Biolegend, 165002, Purified anti-mouse CD115 (CSF-1R) Antibody, 100μg

CSF-1R, also known as CD115 and M-CSFR, is a single-pass type I membrane protein and member of the platelet-derived growth factor receptor family. This c-fms (Fms proto-oncogene) gene product's natural ligands include M-CSF and IL-34. Structural studies of CD115 have described an Ig-like extracellular domain, a transmembrane domain, an intracellular juxtamembrane domain, a split tyrosine kinase domain, and a C-terminal tail receptor. Receptor activation induces homodimerization in addition to phosphorylation and ubiquitination of intracellular residues. CD115 directly influences tissue macrophage and osteoclast differentiation and proliferation. It is expressed on monocytes/macrophages, peritoneal exudate cells, plasmacytoid and conventional dendritic cells, and osteoclasts.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Moue CD115 transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone W19330C does not affect binding of clone W19330E or clone AFS98 on the target.
RRID: AB_2910349 (BioLegend Cat. No. 165002)
Structure: Ig superfamily, 145 kD
Distribution: Monocytes/macrophages, peritoneal exudate cells, plasmacytoid and conventional dendritic cells, osteoclasts
Function: Growth factor receptor, tyrosine kinase
Ligand/Receptor: Macrophage colony stimulating factor (M-CSF), IL-34
Cell Type: Dendritic cells, Macrophages, Monocytes, Osteoclasts
Biology Area: Cell Biology, Immunology, Stem Cells
Molecular Family: Cytokine/Chemokine Receptors
Antigen References: Guilbert LJ and Stanley ER. 1980. J Cell Biol. 85:153-9. Yeung YG, et al. 1978. Proc Natl Acad Sci U S A. 84:1268-71. Sherr CJ, et al. 1985. Cell. 41:665-76.
Gene ID: 12978
UniProt: View information about CD115 on UniProt.org
Clone: W19330C
Regulatory Status: RUO
Other Names: CSF-1R, M-CSR, c-fms
Isotype: Rat IgG2a, λ