Biolegend, 165203, PE anti-mouse CD115 (CSF-1R) Antibody, 25μg

CSF-1R, also known as CD115 and M-CSFR, is a single-pass type I membrane protein and member of the platelet-derived growth factor receptor family. This c-fms (Fms proto-oncogene) gene product's natural ligands include M-CSF and IL-34. Structural studies of CD115 have described an Ig-like extracellular domain, a transmembrane domain, an intracellular juxtamembrane domain, a split tyrosine kinase domain, and a C-terminal tail receptor. Receptor activation induces homodimerization in addition to phosphorylation and ubiquitination of intracellular residues. CD115 directly influences tissue macrophage and osteoclast differentiation and proliferation. It is expressed on monocytes/macrophages, peritoneal exudate cells, plasmacytoid and conventional dendritic cells, and osteoclasts.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse CD115 transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Clone W19330E does not affect binding of clone W19330C on target cells. Clone W19330E can block clone AFS98 binding on target cells. Clone W19330E can block ligand binding to CSFR-1.
RRID: AB_2910352 (BioLegend Cat. No. 165203) AB_2910352 (BioLegend Cat. No. 165204)
Structure: Ig superfamily, 145 kD
Distribution: Monocytes/macrophages, peritoneal exudate cells, plasmacytoid and conventional dendritic cells, osteoclasts
Function: Growth factor receptor, tyrosine kinase
Ligand/Receptor: Macrophage colony stimulating factor (M-CSF), IL-34
Cell Type: Dendritic cells, Macrophages, Monocytes, Osteoclasts
Biology Area: Cell Biology, Immunology, Stem Cells
Molecular Family: Cytokine/Chemokine Receptors
Antigen References: Guilbert LJ and Stanley ER. 1980. J Cell Bio. 85:153-9. Yeung Y-G, et al. 1978. Proc Natl Acad Sci. 84:1268-71. Sherr CJ, et al. 1985. Cell. 41:665-76.
Gene ID: 12978
UniProt: View information about CD115 on UniProt.org
Clone: W19330E
Regulatory Status: RUO
Other Names: CSF-1R, M-CSR, c-fms
Isotype: Rat IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.