Biolegend, 166006, FITC anti-mouse CD52 Antibody, 100μg

CD52, also known as Cambridge pathology antigen 1 (CAMPATH-1), is a 25-29 kD glycoprotein containing a large N-linked carbohydrate moiety. The actual molecule of CD52 is only 8-9 kD. It is expressed in the male reproductive tract and on virtually all lymphocytes (T and B cells), as well as macrophages/monocytes, eosinophils, and red cells. CD52 is thought to play a role in carrying and orienting carbohydrates. CD52 is a potent target for complement-mediated lysis and antibody-mediated cellular cytotoxicity and has been used as a depletion target for chronic lymphocytic leukemia (CLL)/lymphoma and immunosuppression.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
RRID: AB_3675017 (BioLegend Cat. No. 166005) AB_3675017 (BioLegend Cat. No. 166006)
Structure: CD52 is a peptide of 12 amino acids, anchored to glycosylphosphatidylinositol (GPI).
Distribution: Expressed on lymphohematopoietic tissues, including thymus, spleen, and bone marrow, but not in liver, kidney, and brain
Function: Promote cell-mediated killing (antibody-dependent cellular cytotoxicity)
Cell Type: Dendritic cells, Lymphocytes, Mesenchymal Stem Cells, Monocytes, Thymocytes
Biology Area: Cell Adhesion
Molecular Family: Adhesion Molecules
Antigen References: Gudrun R, et al. 2002. Blood. 101:1422.
Gene ID: 23833
UniProt: View information about CD52 on UniProt.org
Clone: S19014A
Regulatory Status: RUO
Other Names: CAMPATH-1 antigen
Isotype: Rat IgM, κ